Rabbit KERA ELISA Kit | KERA elisa kit

Rabbit KERA (Keratocan) ELISA Kit

Cat. Num. AAA2508325

• Gene Names: KERA; KTN; CNA2; SLRR2B
• Reactivity: Rabbit
• Synonyms: KERA; Rabbit KERA (Keratocan) ELISA Kit; KERA elisa kit

• Reactivity: Rabbit
• Specificity: This kit recognizes natural and recombinant Rabbit KERA. No significant cross-reactivity or interference between Rabbit KERA and analogues was observed.
• Sequence Length: 352

• Samples: Serum, Plasma, Biological Fluids
• Assay Type: Sandwich
• Detection Range: 125-8000pg/mL
• Sensitivity: Min: 75pg/mL; Max: 8000pg/mL
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for KERA elisa kit

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Rabbit KERA concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to KERA. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for KERA and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain KERA, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of KERA. You can calculate the concentration of KERA in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 5901992
• NCBI GeneID: 11081
• NCBI Accession #: NP_008966.1
• NCBI GenBank Nucleotide #: NM_007035.3
• UniProt Accession #: O60938
• Molecular Weight: 40,509 Da
• NCBI Official Full Name: keratocan
• NCBI Official Synonym Full Names: keratocan
• NCBI Official Symbol: KERA
• NCBI Official Synonym Symbols: KTN; CNA2; SLRR2B
• NCBI Protein Information: keratocan; keratan sulfate proteoglycan keratocan
• UniProt Protein Name: Keratocan
• Protein Family: Keratin
• UniProt Gene Name: KERA
• UniProt Synonym Gene Names: SLRR2B; KTN
• UniProt Entry Name: KERA_HUMAN

NCBI Description

The protein encoded by this gene is a keratan sulfate proteoglycan that is involved in corneal transparency. Defects in this gene are a cause of autosomal recessive cornea plana 2 (CNA2).[provided by RefSeq, May 2010]

Uniprot Description

KERA: May be important in developing and maintaining corneal transparency and for the structure of the stromal matrix. Defects in KERA are the cause of the autosomal recessive cornea plana 2 (CNA2). In CNA2, the forward convex curvature is flattened, leading to a decrease in refraction, reduced visual activity, extreme hyperopia (usually plus 10 d or more), hazy corneal limbus, opacities in the corneal parenchyma, and marked arcus senilis (often detected at an early age). CNA2 is a rare disorder with a worldwide distribution, but a high prevalence in the Finnish population. Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class II subfamily.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 12q22

Cellular Component: proteinaceous extracellular matrix; lysosomal lumen; Golgi lumen; extracellular region

Biological Process: keratan sulfate metabolic process; visual perception; glycosaminoglycan metabolic process; carbohydrate metabolic process; response to stimulus; keratan sulfate biosynthetic process; pathogenesis; keratan sulfate catabolic process

Disease: Cornea Plana 2