Human Interleukin 24 (IL24) CLIA Kit | IL24 clia kit

Human Interleukin 24 (IL24) CLIA Kit

Cat. Num. AAA2000973

• Gene Names: IL24; C49A; FISP; MDA7; MOB5; ST16; IL10B
• Reactivity: Human
• Synonyms: Interleukin 24 (IL24); Human Interleukin 24 (IL24) CLIA Kit; C49A; FISP; IL10B; MDA7; Mob-5; ST16; Mda-7; Melanoma Differentiation Association Protein 7; Suppression Of Tumorigenicity 16; IL24 clia kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Interleukin 24 (IL24).; No significant cross-reactivity or interference between Interleukin 24 (IL24) and analogues was observed.
• Sequence Length: 207

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma and Other Biological Fluids
• Detection Range: 1.37-1,000pg/mL
• Sensitivity: < 0.57pg/mL
• Application: Chemiluminescent immunoassay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 24 (IL24) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 24 (IL24) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IL24 clia kit

The microplate provided in this kit has been pre-coated with an antibody specific to Interleukin 24 (IL24). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Interleukin 24 (IL24). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Interleukin 24 (IL24) level in the sample or standard.;

Product Categories/Family for IL24 clia kit

Cytokine; Signal transduction; Tumor immunity; Infection immunity; Dermatology

NCBI and Uniprot Product Information

• NCBI GI #: 16307185
• NCBI GeneID: 11009
• UniProt Accession #: Q13007; Q2YHE5; Q53XZ7; Q5YLN8; Q96DB0; Q96KG4
• Molecular Weight: 7,347 Da
• NCBI Official Full Name: Interleukin 24
• NCBI Official Synonym Full Names: interleukin 24
• NCBI Official Symbol: IL24
• NCBI Official Synonym Symbols: C49A; FISP; MDA7; MOB5; ST16; IL10B
• NCBI Protein Information: interleukin-24
• UniProt Protein Name: Interleukin-24
• Protein Family: Interleukin
• UniProt Gene Name: IL24
• UniProt Synonym Gene Names: MDA7; ST16; IL-24; MDA-7
• UniProt Entry Name: IL24_HUMAN

NCBI Description

This gene encodes a member of the IL10 family of cytokines. It was identified as a gene induced during terminal differentiation in melanoma cells. The protein encoded by this gene can induce apoptosis selectively in various cancer cells. Overexpression of this gene leads to elevated expression of several GADD family genes, which correlates with the induction of apoptosis. The phosphorylation of mitogen-activated protein kinase 14 (MAPK7/P38), and heat shock 27kDa protein 1 (HSPB2/HSP27) are found to be induced by this gene in melanoma cells, but not in normal immortal melanocytes. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]

Uniprot Description

IL24: Has antiproliferative properties on melanoma cells and may contribute to terminal cell differentiation. Belongs to the IL-10 family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Motility/polarity/chemotaxis; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 1q32

Cellular Component: extracellular space; endoplasmic reticulum

Molecular Function: cytokine activity

Biological Process: negative regulation of cell proliferation; wound healing; apoptosis; positive regulation of cell proliferation; positive regulation of caspase activity; positive regulation of JAK-STAT cascade; immune response; negative regulation of caspase activity; inflammatory response; negative regulation of cell migration; positive regulation of tyrosine phosphorylation of Stat3 protein; serine phosphorylation of STAT3 protein