Human Interleukin 24 ELISA Kit | IL-24 elisa kit

Human Interleukin 24 ELISA Kit

Cat. Num. AAA760485

• Gene Names: IL24; C49A; FISP; MDA7; MOB5; ST16; IL10B
• Reactivity: Human
• Synonyms: Interleukin 24; Human Interleukin 24 ELISA Kit; IL-24 (Interleukin 24); IL-24 elisa kit

• Reactivity: Human
• Sequence Length: 207

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 15.625-1000pg/ml
• Sensitivity: 9.375pg/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for IL-24 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human IL- 24antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human IL-24 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human IL-24 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration ofHuman IL-24can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 16307185
• NCBI GeneID: 11009
• Molecular Weight: 7,347 Da
• NCBI Official Full Name: Interleukin 24
• NCBI Official Synonym Full Names: interleukin 24
• NCBI Official Symbol: IL24
• NCBI Official Synonym Symbols: C49A; FISP; MDA7; MOB5; ST16; IL10B
• NCBI Protein Information: interleukin-24
• UniProt Protein Name: Interleukin-24
• UniProt Gene Name: IL24
• UniProt Synonym Gene Names: MDA7; ST16; IL-24; MDA-7
• UniProt Entry Name: IL24_HUMAN

NCBI Description

This gene encodes a member of the IL10 family of cytokines. It was identified as a gene induced during terminal differentiation in melanoma cells. The protein encoded by this gene can induce apoptosis selectively in various cancer cells. Overexpression of this gene leads to elevated expression of several GADD family genes, which correlates with the induction of apoptosis. The phosphorylation of mitogen-activated protein kinase 14 (MAPK7/P38), and heat shock 27kDa protein 1 (HSPB2/HSP27) are found to be induced by this gene in melanoma cells, but not in normal immortal melanocytes. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]

Uniprot Description

IL24: Has antiproliferative properties on melanoma cells and may contribute to terminal cell differentiation. Belongs to the IL-10 family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 1q32

Cellular Component: extracellular region; extracellular space

Molecular Function: cytokine activity; protein binding

Biological Process: immune response; inflammatory response; positive regulation of JAK-STAT cascade