Human Chymase ELISA Kit | CMA1 elisa kit

Human Chymase ELISA Kit

Cat. Num. AAA761959

• Gene Names: CMA1; CYH; MCT1; chymase
• Reactivity: Human
• Synonyms: Chymase; Human Chymase ELISA Kit; CMA1 (Chymase)/Alpha-chymase/Mast cell protease I; CMA1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of CMA1. No significant cross-reactivity or interference between CMA1 and analogues was observed.
• Sequence Length: 247

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.312-20ng/ml
• Sensitivity: 0.188ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level CMA1 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level CMA1 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CMA1 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- CMA1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- CMA1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the CMA1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of CMA1 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 4502907
• NCBI GeneID: 1215
• NCBI Accession #: NP_001827.1
• NCBI GenBank Nucleotide #: NM_001836.4
• Molecular Weight: 14,893 Da
• NCBI Official Full Name: chymase isoform 1 preproprotein
• NCBI Official Synonym Full Names: chymase 1, mast cell
• NCBI Official Symbol: CMA1
• NCBI Official Synonym Symbols: CYH; MCT1; chymase
• NCBI Protein Information: chymase
• UniProt Protein Name: Chymase
• Protein Family: Chymase
• UniProt Gene Name: CMA1
• UniProt Synonym Gene Names: CYH; CYM
• UniProt Entry Name: CMA1_HUMAN

NCBI Description

This gene encodes a chymotryptic serine proteinase that belongs to the peptidase family S1. It is expressed in mast cells and is thought to function in the degradation of the extracellular matrix, the regulation of submucosal gland secretion, and the generation of vasoactive peptides. In the heart and blood vessels, this protein, rather than angiotensin converting enzyme, is largely responsible for converting angiotensin I to the vasoactive peptide angiotensin II. Alternative splicing results in multiple variants. [provided by RefSeq, Apr 2015]

Uniprot Description

CMA1: Major secreted protease of mast cells with suspected roles in vasoactive peptide generation, extracellular matrix degradation, and regulation of gland secretion. Belongs to the peptidase S1 family. Granzyme subfamily.

Protein type: Secreted, signal peptide; Secreted; Protease; EC 3.4.21.39

Chromosomal Location of Human Ortholog: 14q11.2

Cellular Component: cytoplasm; extracellular matrix; extracellular region; intracellular membrane-bound organelle

Molecular Function: peptide binding; serine-type endopeptidase activity; serine-type peptidase activity

Biological Process: angiotensin maturation; cellular protein metabolic process; extracellular matrix disassembly; extracellular matrix organization and biogenesis; immune response; interleukin-1 beta biosynthetic process; midbrain development; positive regulation of angiogenesis; protein processing; regulation of inflammatory response