Mouse anti-Human NR2C2 / TAK1 Monoclonal Antibody | anti-NR2C2 antibody

Mouse Monoclonal [clone 2A5] (IgG1,k) to Human NR2C2 / TAK1

Cat. Num. AAA245667

• Gene Names: NR2C2; TR4; TAK1
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Protein A Purified
• Synonyms: NR2C2 / TAK1; Monoclonal Antibody; Mouse Monoclonal [clone 2A5] (IgG1; k) to Human NR2C2 / TAK1; Anti-NR2C2 / TAK1 Antibody (clone 2A5) IHC-plus; NR2C2; Nuclear hormone receptor TR4; Nuclear receptor TAK1; Orphan nuclear receptor TR4; Orphan nuclear receptor TAK1; Testicular orphan receptor-4; TR2R1; TR4; Testicular nuclear receptor 4; Testicular receptor 4; TR4 nuclear hormone receptor; Human NR2C2; TAK1; anti-NR2C2 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 2A5
• Specificity: Human NR2C2
• Purity/Purification: Protein A Purified
• Form/Format: PBS, pH 7.4
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 615

• Applicable Applications for anti-NR2C2 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA, IHC-P (5 ug/ml), WB; Usage: Immunohistochemistry: Formalin-fixed paraffin-embedded sections. RNAi Knockdown: Antibody validated. Western Blot using transfected cell lysates and the recombinant protein used as the immunogen.
• Target Species: Human
• Immunogen Description: NR2C2 (NP_003289, 43 a.a. ~ 153 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Immunogen: NR2C2 / TAK1 antibody was raised against nR2C2 (NP_003289, 43 a.a. ~ 153 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Preparation and Storage: Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.

Immunohistochemistry (IHC)

(Anti-NR2C2 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Western Blot (WB)

(Western blot of NR2C2 expression in transfected 293T cell line by NR2C2 monoclonal antibody clone 2A5.)

Related Product Information for anti-NR2C2 antibody

TR4 is a NR2 Hepatocyte NF4-Like nuclear hormone receptor and transcription factor related to the orphan receptors TR2, COUP-TFI, and ARP-1. TR4 has been shown to affect the proliferation and maturation of brain structures, neurogenesis, the regulation of myeloid progenitor cell proliferation and development, and retinoid-mediated signaling pathways.

Product Categories/Family for anti-NR2C2 antibody

Family: NHR; Subfamily: NR2 Hepatocyte NF4-like

NCBI and Uniprot Product Information

• NCBI GI #: 36950991
• NCBI GeneID: 7182
• NCBI Accession #: NP_003289.2
• NCBI GenBank Nucleotide #: NM_003298.4
• UniProt Accession #: P49116; P55092; A8K3H5; B6ZGT8
• Molecular Weight: 67,345 Da
• NCBI Official Full Name: nuclear receptor subfamily 2 group C member 2 isoform 1
• NCBI Official Synonym Full Names: nuclear receptor subfamily 2, group C, member 2
• NCBI Official Symbol: NR2C2
• NCBI Official Synonym Symbols: TR4; TAK1
• NCBI Protein Information: nuclear receptor subfamily 2 group C member 2; orphan nuclear receptor TR4; Nuclear hormone receptor TR4; orphan nuclear receptor TAK1; testicular nuclear receptor 4
• UniProt Protein Name: Nuclear receptor subfamily 2 group C member 2
• Protein Family: Nuclear receptor subfamily
• UniProt Gene Name: NR2C2
• UniProt Synonym Gene Names: TAK1; TR4
• UniProt Entry Name: NR2C2_HUMAN

NCBI Description

This gene encodes a protein that belongs to the nuclear hormone receptor family. Members of this family act as ligand-activated transcription factors and function in many biological processes such as development, cellular differentiation and homeostasis. The activated receptor/ligand complex is translocated to the nucleus where it binds to hormone response elements of target genes. The protein encoded by this gene plays a role in protecting cells from oxidative stress and damage induced by ionizing radiation. The lack of a similar gene in mouse results in growth retardation, severe spinal curvature, subfertility, premature aging, and prostatic intraepithelial neoplasia (PIN) development. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Apr 2014]

Uniprot Description

NR2C2: Orphan nuclear receptor that can act as a repressor or activator of transcription. An important repressor of nuclear recptor signaling pathways such as retinoic acid receptor, retinoid X, vitamin D3 receptor, thyroid hormone receptor and estrogen receptor pathways. May regulate gene expression during the late phase of spermatogenesis. Together with NR2C1, forms the core of the DRED (direct repeat erythroid-definitive) complex that represses embryonic and fetal globin transcription including that of GATA1. Binds to hormone response elements (HREs) consisting of two 5'-AGGTCA-3' half site direct repeat consensus sequences. Plays a fundamental role in early embryonic development and embryonic stem cells. Required for normal spermatogenesis and cerebellum development. Appears to be important for neurodevelopmentally regulated behavior. Activates transcriptional activity of LHCG. Antagonist of PPARA-mediated transactivation. Belongs to the nuclear hormone receptor family. NR2 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Transcription factor; Nuclear receptor

Chromosomal Location of Human Ortholog: 3p25

Cellular Component: nucleoplasm

Molecular Function: protein binding; zinc ion binding; protein heterodimerization activity; sequence-specific DNA binding; transcription coactivator activity; steroid hormone receptor activity; receptor activity; transcription factor activity

Biological Process: nervous system development; transcription initiation from RNA polymerase II promoter; positive regulation of embryonic development; regulation of transcription, DNA-dependent; meiotic cell cycle; positive regulation of behavior; cerebellum development; steroid hormone mediated signaling; gene expression; spermatogenesis; positive regulation of transcription from RNA polymerase II promoter; cell differentiation