Human INS (Insulin) CLIA Kit | INS clia kit

Human INS (Insulin) CLIA Kit

Cat. Num. AAA2531542

• Gene Names: INS; IDDM; ILPR; IRDN; IDDM1; IDDM2; MODY10
• Reactivity: Human
• Synonyms: INS (Insulin); Human INS (Insulin) CLIA Kit; IDDM2; ILPR; IRDN; MODY10; INS clia kit

• Reactivity: Human
• Specificity: This kit recognizes natural and recombinant INS. No significant cross-reactivity or interference between INS and analogues was observed.
• Sequence Length: 110

• Assay Type: Sandwich
• Detection Range: 0.625-40ng/mL
• Sensitivity: Min: 0.375ng/mL; Max: 40ng/mL

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for INS clia kit

Intended Uses: This CLIA kit applies to the in vitro quantitative determination of INS concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to INS. Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for INS and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain INS, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of INS. You can calculate the concentration of INS in the samples by comparing the RLU value of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 386828
• NCBI GeneID: 3630
• Molecular Weight: 21,537 Da
• NCBI Official Full Name: insulin
• NCBI Official Synonym Full Names: insulin
• NCBI Official Symbol: INS
• NCBI Official Synonym Symbols: IDDM; ILPR; IRDN; IDDM1; IDDM2; MODY10
• NCBI Protein Information: insulin
• UniProt Protein Name: Insulin
• Protein Family: Insulin
• UniProt Gene Name: INS
• UniProt Entry Name: INS_HUMAN

NCBI Description

After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into three peptides: the B chain and A chain peptides, which are covalently linked via two disulfide bonds to form insulin, and C-peptide. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. A multitude of mutant alleles with phenotypic effects have been identified. There is a read-through gene, INS-IGF2, which overlaps with this gene at the 5' region and with the IGF2 gene at the 3' region. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2010]

Uniprot Description

Insulin: Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver. Heterodimer of a B chain and an A chain linked by two disulfide bonds. Belongs to the insulin family.

Protein type: Secreted, signal peptide; Secreted; Hormone

Chromosomal Location of Human Ortholog: 11p15.5

Cellular Component: extracellular space; endoplasmic reticulum lumen; Golgi lumen; extracellular region

Molecular Function: identical protein binding; insulin-like growth factor receptor binding; protein binding; protease binding; hormone activity; insulin receptor binding

Biological Process: positive regulation of nitric oxide biosynthetic process; positive regulation of vasodilation; glucose homeostasis; activation of NF-kappaB transcription factor; positive regulation of glucose import; negative regulation of protein oligomerization; regulation of protein localization; cell-cell signaling; negative regulation of gluconeogenesis; acute-phase response; regulation of transmembrane transporter activity; negative regulation of NAD(P)H oxidase activity; positive regulation of mitosis; activation of protein kinase B; positive regulation of nitric-oxide synthase activity; negative regulation of acute inflammatory response; glucose metabolic process; positive regulation of lipid biosynthetic process; positive regulation of cell growth; positive regulation of protein amino acid autophosphorylation; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of peptidyl-tyrosine phosphorylation; negative regulation of protein catabolic process; positive regulation of cell differentiation; regulation of amino acid metabolic process; negative regulation of glycogen catabolic process; wound healing; positive regulation of glycogen biosynthetic process; positive regulation of cellular protein metabolic process; glucose transport; regulation of protein secretion; positive regulation of MAPKKK cascade; regulation of transcription, DNA-dependent; negative regulation of vasodilation; negative regulation of lipid catabolic process; positive regulation of cell proliferation; negative regulation of proteolysis; negative regulation of protein secretion; MAPKKK cascade; alpha-beta T cell activation; negative regulation of fatty acid metabolic process; endocrine pancreas development; G-protein coupled receptor protein signaling pathway; positive regulation of protein kinase B signaling cascade; cellular protein metabolic process; fatty acid homeostasis; positive regulation of glycolysis; insulin receptor signaling pathway; energy reserve metabolic process; positive regulation of insulin receptor signaling pathway; regulation of insulin secretion; positive regulation of DNA replication; positive regulation of cytokine secretion; positive regulation of cell migration

Disease: Diabetes Mellitus, Insulin-dependent, 2; Diabetes Mellitus, Permanent Neonatal; Hyperproinsulinemia; Maturity-onset Diabetes Of The Young, Type 10