Western Blot (WB)
(CBX4 Antibody (N-term) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the CBX4 antibody detected the CBX4 protein (arrow).)
Rabbit anti-Human CBX4 Polyclonal Antibody | anti-CBX4 antibody
CBX4, NT (CBX4, E3 SUMO-protein ligase CBX4, Chromobox protein homolog 4, Polycomb 2 homolog) (APC)
Gene Names
CBX4; PC2; NBP16
Reactivity
Human
Applications
Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
CBX4; Polyclonal Antibody; NT (CBX4; E3 SUMO-protein ligase CBX4; Chromobox protein homolog 4; Polycomb 2 homolog) (APC); Polycomb 2 homolog; anti-CBX4 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
Applicable Applications for anti-CBX4 antibody
Western Blot (WB), FLISA
Application Notes
WB: 1:100-500
FLISA: 1:1,000
Applications are based on unconjugated antibody.
FLISA: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
CBX4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human CBX4.
Conjugate
APC
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(CBX4 Antibody (N-term) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the CBX4 antibody detected the CBX4 protein (arrow).)
Western Blot (WB)
(CBX4 Antibody (N-term) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the CBX4 antibody detected the CBX4 protein (arrow).)
Related Product Information for anti-CBX4 antibody
E3 SUMO-protein ligase which facilitates SUMO1 conjugation by UBE2I. Component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility.
Product Categories/Family for anti-CBX4 antibody
References
Seong, I.S., et al. Hum. Mol. Genet. 19(4):573-583(2010). Zhou, W., et al. BMB Rep 42(3):154-159(2009). Liang, G., et al. Hum. Mol. Genet. 17(8):1109-1119(2008). Kim, S.H., et al. Int. J. Biochem. Cell Biol. 40(11):2462-2471(2008). Kabil, O., et al. Biochemistry 45(45):13528-13536(2006).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
31,987 Da
NCBI Official Full Name
E3 SUMO-protein ligase CBX4
NCBI Official Synonym Full Names
chromobox homolog 4
NCBI Official Symbol
CBX4
NCBI Official Synonym Symbols
PC2; NBP16
NCBI Protein Information
E3 SUMO-protein ligase CBX4; Pc class 2 homolog; polycomb 2 homolog; NS5ATP1-binding protein 16; chromobox protein homolog 4; chromobox homolog 4 (Pc class homolog, Drosophila)
UniProt Protein Name
E3 SUMO-protein ligase CBX4
Protein Family
UniProt Gene Name
CBX4
UniProt Synonym Gene Names
Pc2; hPc2
UniProt Entry Name
CBX4_HUMAN
Uniprot Description
CBX4: E3 SUMO-protein ligase which facilitates SUMO1 conjugation by UBE2I. Involved in the sumoylation of HNRNPK, a p53/TP53 transcriptional coactivator, hence indirectly regulates p53/TP53 transcriptional activation resulting in p21/CDKN1A expression. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Transcription, coactivator/corepressor; SUMO ligase; EC 6.3.2.-
Chromosomal Location of Human Ortholog: 17q25.3
Cellular Component: nucleoplasm; nuclear speck; PcG protein complex; nucleus
Molecular Function: protein binding; enzyme binding; single-stranded RNA binding; SUMO binding; phosphoprotein binding; chromatin binding; transcription corepressor activity; methylated histone residue binding; ligase activity
Biological Process: protein sumoylation; transcription, DNA-dependent; negative regulation of transcription from RNA polymerase II promoter; chromatin modification; negative regulation of transcription, DNA-dependent; negative regulation of apoptosis
Research Articles on CBX4
1. The presence of CBX4-GFP in the same focus had a minor impact on BMI1 and RING1 recovery kinetics.
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Product Notes
The CBX4 cbx4 (Catalog #AAA6281212) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CBX4, NT (CBX4, E3 SUMO-protein ligase CBX4, Chromobox protein homolog 4, Polycomb 2 homolog) (APC) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CBX4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), FLISA. WB: 1:100-500 FLISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the CBX4 cbx4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CBX4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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