Rabbit IRX3 Polyclonal Antibody | anti-IRX3 antibody

IRX3, NT (IRX3, IRXB1, Iroquois-class homeodomain protein IRX-3, Homeodomain protein IRXB1, Iroquois homeobox protein 3) (MaxLight 405)

Cat. Num. AAA6310987

• Gene Names: IRX3; IRX-1; IRXB1
• Reactivity: Human, Xenopus
• Applications: Western Blot, Immunohistochemistry
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: IRX3; Polyclonal Antibody; NT (IRX3; IRXB1; Iroquois-class homeodomain protein IRX-3; Homeodomain protein IRXB1; Iroquois homeobox protein 3) (MaxLight 405); Iroquois homeobox protein 3; anti-IRX3 antibody

• Host: Rabbit
• Reactivity: Human, Xenopus
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human IRX3. Species Crossreactivity: Xenopus
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight405.

• Applicable Applications for anti-IRX3 antibody: Western Blot (WB), Immunohistochemistry (IHC), FLISA
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 111-140 in the N-terminal region of human IRX3, UniProt Accession #NP_077312.2; P78415; O42261; P81067.
• Conjugate: MaxLight405
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight405 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in 293T, SH-SY5Y cell line lysates (35ug/lane) usingMBS6002100. This demonstrates the IRX3 antibody detected the IRX3 protein (arrow).)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma using MBS6002100 followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of MBS6002100 for immunohistochemistry.)

Related Product Information for anti-IRX3 antibody

IRX3 is a member of the Iroquois homeobox gene family (see IRX1; MIM 606197) and plays a role in an early step of neural development (Bellefroid et al., 1998 [PubMed 9427753]). Members of this family appear to play multiple roles during pattern formation of vertebrate embryos (Lewis et al., 1999 [PubMed 10370142]).

Product Categories/Family for anti-IRX3 antibody

Antibodies; Transcription Factors; Zinc; Ring; Homeobox

References

1. Ragvin, A., et al. Proc. Natl. Acad. Sci. U.S.A. 107(2):775-780 (2010). 2. Trynka, G., et al. Gut 58(8):1078-1083 (2009). 3. Lewis, M.T., et al. Cell Tissue Res. 296(3):549-554 (1999). 4. Bellefroid, E.J., et al. EMBO J. 17(1):191-203 (1998).

NCBI and Uniprot Product Information

• NCBI GI #: 226371735
• NCBI GeneID: 79191
• NCBI Accession #: NP_077312.2
• NCBI GenBank Nucleotide #: NM_024336.2
• UniProt Accession #: P78415; O42261; P81067
• Molecular Weight: 52,119 Da
• NCBI Official Full Name: iroquois-class homeodomain protein IRX-3
• NCBI Official Synonym Full Names: iroquois homeobox 3
• NCBI Official Symbol: IRX3
• NCBI Official Synonym Symbols: IRX-1; IRXB1
• NCBI Protein Information: iroquois-class homeodomain protein IRX-3; homeodomain protein IRXB1; iroquois homeobox protein 3; iroquois-class homeodomain protein IRX-1
• UniProt Protein Name: Iroquois-class homeodomain protein IRX-3
• Protein Family: Iroquois-class homeodomain protein
• UniProt Gene Name: IRX3
• UniProt Synonym Gene Names: IRXB1
• UniProt Entry Name: IRX3_HUMAN

NCBI Description

IRX3 is a member of the Iroquois homeobox gene family (see IRX1; MIM 606197) and plays a role in an early step of neural development (Bellefroid et al., 1998 [PubMed 9427753]). Members of this family appear to play multiple roles during pattern formation of vertebrate embryos (Lewis et al., 1999 [PubMed 10370142]).[supplied by OMIM, Aug 2009]

Uniprot Description

IRX3: Transcription factor. Involved in SHH-dependent neural patterning. Together with NKX2-2 and NKX6-1 acts to restrict the generation of motor neurons to the appropriate region of the neural tube. Belongs to the class I proteins of neuronal progenitor factors, which are repressed by SHH signals. Involved in the transcriptional repression of MNX1 in non-motor neuron cells. Belongs to the TALE/IRO homeobox family.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 16q12.2

Cellular Component: axon; cytoplasm; nucleus

Molecular Function: sequence-specific DNA binding

Biological Process: negative regulation of neuron differentiation; regulation of transcription, DNA-dependent; transcription, DNA-dependent; mesoderm development; positive regulation of neuron differentiation; metanephros development