SLAM Family Member 7 Active Protein | Slamf7 active protein

Recombinant Mouse SLAM Family Member 7 Protein

Cat. Num. AAA2556792

• Gene Names: Slamf7; 19A; CS1; 19A24; CRACC; 4930560D03Rik
• Purity: >95% as determined by reducing SDS-PAGE.
• Synonyms: SLAM Family Member 7; Recombinant Mouse SLAM Family Member 7 Protein; SLAM family member 7; Leukocyte cell-surface antigen; Novel Ly9; CD319; Slamf7; CRACC; Slamf7 active protein

• Host: Human Cells
• Purity/Purification: >95% as determined by reducing SDS-PAGE.
• Form/Format: Lyophilized from a 0.2 mum filtered solution of PBS, pH 7.4.
• Sequence Length: 333

• Species: Mouse
• Endotoxin: <1.0 EU per mug as determined by LAL test.
• Fragment: Met 1-Gly 224
• Fusion Tag: C-6His
• Activity: Measured by its ability to bind biotinylated mouse SLAMF7 in a functional ELISA.
• Preparation and Storage: Lyophilized protein should be stored at < -20 degree C, though stable at room temperature for 3 weeks.; Reconstituted protein solution can be stored at 4-7 degree C for 2-7 days.; Aliquots of reconstituted samples are stable at < -20 degree C for 3 months.

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Related Product Information for Slamf7 active protein

Protein Construction: Recombinant Mouse SLAM Family Member 7 is produced by our Mammalian expression system and the target gene encoding Ser23-Gly224 is expressed with a 6His tag at the C-terminus.

Background: SLAM family member 7/CRACC is a type I transmembrane glycoprotein in the SLAM subgroup of the CD2 family. SLAM receptors triggered by homo- or heterotypic cell-cell interactions are modulating the activation and differentiation of a wide variety of immune cells and thus are involved in the regulation and interconnection of both innate and adaptive immune response. Activities are controlled by presence or absence of small cytoplasmic adapter proteins, SH2D1A/SAP and/or SH2D1B/EAT-2. Mature mouse CRACC consists of a 202 amino acid extracellular domain (ECD) with one Ig-like V-set domain and one Ig-like C2-set domain, a 21 aa transmembrane segment, and an 88 aa cytoplasmic domain with two immunoreceptor tyrosine-based switch motifs ITSMs. CRACC is expressed on the surface of NK cells, CD8+ T cells, activated B cells, and mature dendritic cells. It interacts homophilically to induce NK, CTL, and B cell activation.

NCBI and Uniprot Product Information

• NCBI GI #: 31541977
• NCBI GeneID: 75345
• NCBI Accession #: NP_653122.2
• NCBI GenBank Nucleotide #: NM_144539.5
• UniProt Accession #: Q8BHK6; Q8BTL2; Q8CJ63; Q8CJ64; Q8CJ65; Q91XA0
• Molecular Weight: Molecular Mass: 20.1kDa; Actual Protein Molecular Mass: 55-60kDa
• NCBI Official Full Name: SLAM family member 7 isoform 1
• NCBI Official Synonym Full Names: SLAM family member 7
• NCBI Official Symbol: Slamf7
• NCBI Official Synonym Symbols: 19A; CS1; 19A24; CRACC; 4930560D03Rik
• NCBI Protein Information: SLAM family member 7
• UniProt Protein Name: SLAM family member 7
• Protein Family: SLAM family
• UniProt Gene Name: Slamf7

Uniprot Description

Self-ligand receptor of the signaling lymphocytic activation molecule (SLAM) family. SLAM receptors triggered by homo- or heterotypic cell-cell interactions are modulating the activation and differentiation of a wide variety of immune cells and thus are involved in the regulation and interconnection of both innate and adaptive immune response. Activities are controlled by presence or absence of small cytoplasmic adapter proteins, SH2D1A/SAP and/or SH2D1B/EAT-2 (PubMed:19648922). Mediates natural killer (NK) cell activation through a SH2D1A-independent extracellular signal-regulated ERK-mediated pathway (). Positively regulates NK cell functions by a mechanism dependent on the adapter SH2D1B. In addition to heterotypic NK cells-target cells interactions also homotypic interactions between NK cells may contribute to activation. However, in the absence of SH2D1B, inhibits NK cell function. Acts also inhibitory in T-cells (PubMed:19151721). May play a role in lymphocyte adhesion (). In LPS-activated monocytes negatively regulates production of proinflammatory cytokines ().