Rabbit CUL3 Polyclonal Antibody | anti-CUL3 antibody

CUL3 (Cullin-3, CUL-3, PHA2E) (HRP)

Cat. Num. AAA6413113

• Gene Names: CUL3; CUL-3; PHA2E
• Reactivity: Human, Mouse, Rat
• Applications: Immunofluorescence, Western Blot, Immunohistochemistry
• Purity: Purified by affinity chromatography.
• Synonyms: CUL3; Polyclonal Antibody; CUL3 (Cullin-3; CUL-3; PHA2E) (HRP); anti-CUL3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CUL3. Species Crossreactivity: mouse and rat
• Purity/Purification: Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-CUL3 antibody: Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 569-768 of human CUL3.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using 210340 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10,000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit Exposure time: 90s.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human prostate using 210340 at dilution of 1:100 (40x lens).)

Related Product Information for anti-CUL3 antibody

CUL3 (Cullin-3) is a member of the cullin-based ubiquitin ligase family. By interacting with Hrt1 and BTB domain containing proteins, the complex functions as a CUL3-based E3 ligase to bring specific substrates to ubiquitinylation and degradation (1). The CUL3 complex has been shown to target many substrates involved in cell cycle progression (2), transcription (3), development and differentiation (4,5).

Product Categories/Family for anti-CUL3 antibody

Antibodies; Cullin

NCBI and Uniprot Product Information

• NCBI GI #: 12643396
• NCBI GeneID: 8452
• UniProt Accession #: Q13618; O75415; Q569L3; Q9UBI8; Q9UET7; A8K536; B8ZZC3
• Molecular Weight: 768
• NCBI Official Full Name: Cullin-3
• NCBI Official Synonym Full Names: cullin 3
• NCBI Official Symbol: CUL3
• NCBI Official Synonym Symbols: CUL-3; PHA2E
• NCBI Protein Information: cullin-3
• UniProt Protein Name: Cullin-3
• Protein Family: Cullin
• UniProt Gene Name: CUL3
• UniProt Synonym Gene Names: KIAA0617; CUL-3
• UniProt Entry Name: CUL3_HUMAN

NCBI Description

This gene encodes a member of the cullin protein family. The encoded protein plays a critical role in the polyubiquitination and subsequent degradation of specific protein substrates as the core component and scaffold protein of an E3 ubiquitin ligase complex. Complexes including the encoded protein may also play a role in late endosome maturation. Mutations in this gene are a cause of type 2E pseudohypoaldosteronism. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Mar 2012]

Uniprot Description

CUL3: a core component of multiple cullin-RING-based BCR (BTB- CUL3-RBX1) E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with CAND1. The functional specificity of the BCR complex depends on the BTB domain-containing protein as the susbstrate recognition component. SPOP is involved in ubiquitination of BMI1, H2AFY and DAXX, and probably GLI2 or GLI3. BCR(KLHL9-KLHL13) controls the dynamic behavior of AURKB on mitotic chromosomes and thereby coordinates faithful mitotic progression and completion of cytokinesis. BCR(KLHL12) is involved in ER-Golgi transport by regulating the size of COPII coats, thereby playing a key role in collagen export, which is required for embryonic stem (ES) cells division: BCR(KLHL12) acts by mediating monoubiquitination of SEC31A or -B. BCR(KLHL3) acts as a regulator of ion transport in the distal nephron; possibly by mediating ubiquitination of SLC12A3/NCC. Involved in ubiquitination of cyclin E and of cyclin D1 (in vitro) thus involved in regulation of G1/S transition. Forms neddylation-dependent homodimers. Component of multiple BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complexes formed of CUL3, RBX1 and a variable BTB domain-containing protein acting as both, adapter to cullin and substrate recognition subunit. The BCR complex may be active as a heterodimeric complex, in which NEDD8, covalently attached to one CUL3 molecule, binds to the C-terminus of a second CUL3 molecule. Interacts with RBX1, RNF7, CYCE and CAND1. Part of the BCR(SPOP) containing SPOP. Part of the probable BCR(KLHL9-KLHL13) complex with BTB domain proteins KLHL9 and KLHL13. Part of the BCR(KBTBD10) complex containing KBTBD10. Component of the BCR(KLHL12) E3 ubiquitin ligase complex, at least composed of CUL3 and KLHL12 and RBX1. Component of the BCR(KLHL3) E3 ubiquitin ligase complex, at least composed of CUL3 and KLHL3 and RBX1 (Probable). Part of the BCR(ENC1) complex containing ENC1. Part of a complex consisting of BMI1, CUL3 and SPOP. Part of a complex consisting of H2AFY, CUL3 and SPOP. Interacts with KCTD5, KLHL9, KLHL13, GAN, ZBTB16, KLHL21, KLHL3, KLHL15, KLHL20, C16orf44, GMCL1P1, BTBD1. Part of a complex that contains CUL3, RBX1 and GAN. Interacts (via BTB domain) with KLHL17; the interaction regulates surface GRIK2 expression. Widely expressed. Belongs to the cullin family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell cycle regulation; Ubiquitin ligase; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 2q36.2

Cellular Component: Golgi membrane; nucleoplasm; membrane; polar microtubule; cytosol

Molecular Function: protein binding; cyclin binding; protein homodimerization activity; protein heterodimerization activity; ubiquitin protein ligase binding; ubiquitin-protein ligase activity; POZ domain binding

Biological Process: protein monoubiquitination; positive regulation of cytokinesis; integrin-mediated signaling pathway; proteasomal ubiquitin-dependent protein catabolic process; ER to Golgi vesicle-mediated transport; cell migration; Wnt receptor signaling pathway; protein polyubiquitination; stem cell division; protein ubiquitination; gastrulation; mitotic metaphase plate congression; embryonic cleavage; cyclin catabolic process; COPII coating of Golgi vesicle; negative regulation of Rho protein signal transduction; positive regulation of cell proliferation; stress fiber formation; positive regulation of mitotic metaphase/anaphase transition; trophectodermal cellular morphogenesis; cell cycle arrest; G1/S transition of mitotic cell cycle

Disease: Pseudohypoaldosteronism, Type Iie