Signal Transducer CD24/CD24 Active Protein | CD24/CD24 active protein

Recombinant Human Signal Transducer CD24/CD24 (C-mFC)

Cat. Num. AAA2569132

• Gene Names: CD24; CD24A
• Purity: >95% as determined by reducing SDS-PAGE
• Synonyms: Signal Transducer CD24/CD24; Recombinant Human Signal Transducer CD24/CD24 (C-mFC); Signal transducer CD24; Small cell lung carcinoma cluster 4 antigen; CD24; CD24A; CD24/CD24 active protein

• Host: Human Cells
• Purity/Purification: >95% as determined by reducing SDS-PAGE
• Form/Format: Lyophilized from a 0.2um filtered solution of PBS, pH7.4.
• Sequence: Ser27-Gly59

• Species: Human
• Tag: C-mFc
• Endotoxin: <1.0EU per ug as determined by the LAL method.
• Protein Construction: Recombinant Human Signal Transducer CD24 is produced by our Mammalian expression system and the target gene encoding Ser27-Gly59 is expressed with a mFc tag at the C-terminus.
• Activity: Immobilized Human CD24-mFc at 2ug/ml (100ul/well) can bind Anti-Human CD24 mAb . The ED50 of Anti-Human CD24 mAb is 10.1ng/ml.
• Preparation and Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80 degree C. Reconstituted protein solution can be stored at 4-8 degree C for 2-7 days. Aliquots of reconstituted samples are stable at < -20 degree C for 3 months.; This product is provided as lyophilized powder which is shipped with ice packs.

SDS-Page

Activity

Related Product Information for CD24/CD24 active protein

Signal Transducer CD24 is a heavily and variably glycosylated GPI-linked sialoprotein. Human CD24 is expressed on B lineage cells and granulocytes, on epithelial, neuronal, and muscle cells, and on a range of tumor cells. CD24 expression is regulated during lineage development and with the activation of various cell types. Antibody crosslinking of CD24 enhances the induction of apoptosis in B and T lymphocytes which contributes to negative selection and the induction of immune tolerance. CD24 on antigen presenting cells cooperates with B7 molecules in the costimulation of T cells. CD24 associates in cis with Siglec10 and with the danger-associated molecules HMGB1, HSP70, or HSP90 which are released from necrotic or damaged cells. Formation of these ternary complexes fills a protective role: the resulting Siglec10 signaling inhibits inflammatory responses that are otherwise induced by extracellular DAMPs.

Product Categories/Family for CD24/CD24 active protein

Neuroscience; Cancer; immunology; metabolism; Stem cells

NCBI and Uniprot Product Information

• NCBI GI #: 143811372
• NCBI GeneID: 100133941
• UniProt Accession #: P25063; Q16257; Q53XS0
• Molecular Weight: 80
• NCBI Official Full Name: Signal transducer CD24
• NCBI Official Synonym Full Names: CD24 molecule
• NCBI Official Symbol: CD24
• NCBI Official Synonym Symbols: CD24A
• NCBI Protein Information: signal transducer CD24; CD24 antigen (small cell lung carcinoma cluster 4 antigen)
• UniProt Protein Name: Signal transducer CD24
• UniProt Gene Name: CD24
• UniProt Synonym Gene Names: CD24A
• UniProt Entry Name: CD24_HUMAN

NCBI Description

This gene encodes a sialoglycoprotein that is expressed on mature granulocytes and B cells and modulates growth and differentiation signals to these cells. The precursor protein is cleaved to a short 32 amino acid mature peptide which is anchored via a glycosyl phosphatidylinositol (GPI) link to the cell surface. This gene was missing from previous genome assemblies, but is properly located on chromosome 6. Non-transcribed pseudogenes have been designated on chromosomes 1, 15, 20, and Y. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2014]

Uniprot Description

CD24: Modulates B-cell activation responses. Signaling could be triggered by the binding of a lectin-like ligand to the CD24 carbohydrates, and transduced by the release of second messengers derived from the GPI-anchor. Promotes AG-dependent proliferation of B-cells, and prevents their terminal differentiation into antibody-forming cells. Genetic variations in CD24 are associated with susceptibility to multiple sclerosis (MS). A multifactorial, inflammatory, demyelinating disease of the central nervous system. Sclerotic lesions are characterized by perivascular infiltration of monocytes and lymphocytes and appear as indurated areas in pathologic specimens (sclerosis in plaques). The pathological mechanism is regarded as an autoimmune attack of the myelin sheat, mediated by both cellular and humoral immunity. Clinical manifestations include visual loss, extra-ocular movement disorders, paresthesias, loss of sensation, weakness, dysarthria, spasticity, ataxia and bladder dysfunction. Genetic and environmental factors influence susceptibility to the disease. Polymorphisms in CD24 may act as a genetic modifier for susceptibility and progression of MS in some populations, perhaps by affecting the efficiency of CD24 expression on the cell surface. Belongs to the CD24 family.

Protein type: Membrane protein, GPI anchor; Cell surface; Cell development/differentiation; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 6q21

Cellular Component: cell surface; membrane; plasma membrane; lipid raft

Molecular Function: protein binding; signal transducer activity; protein tyrosine kinase activator activity; protein kinase binding

Biological Process: respiratory burst; axon guidance; cell migration; Wnt receptor signaling pathway; regulation of phosphorylation; B cell receptor transport into lipid raft; immune response-regulating cell surface receptor signaling pathway; chemokine receptor transport out of lipid raft; positive regulation of activated T cell proliferation; positive regulation of MAP kinase activity; cell activation; cholesterol homeostasis; cell-cell adhesion; elevation of cytosolic calcium ion concentration; response to estrogen stimulus; response to molecule of bacterial origin; regulation of MAPKKK cascade; negative regulation of transforming growth factor-beta3 production; T cell costimulation; regulation of epithelial cell differentiation; response to hypoxia; regulation of cytokine and chemokine mediated signaling pathway

Disease: Multiple Sclerosis, Susceptibility To