Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Shadow of prion protein (SPRN) Standard Curve.)
Mouse Shadow Of Prion Protein (SPRN) ELISA Kit | SPRN elisa kit
Mouse Shadow Of Prion Protein (SPRN) ELISA Kit
Gene Names
Sprn; C630041J07
Reactivity
Mouse
Synonyms
Shadow Of Prion Protein (SPRN); Mouse Shadow Of Prion Protein (SPRN) ELISA Kit; SPRN; FLJ41197; SHADOO; SHO; bA108K14.1; shadow of prion protein; SPRN elisa kit
Reactivity
Mouse
Specificity
Mouse Shadow of prion protein (SPRN) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse SPRN. No significant cross-reactivity or interference between Mouse SPRN and analogues was observed.
Sequence Length
147
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Shadow of prion protein (SPRN) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Shadow of prion protein (SPRN) Standard Curve.)
Related Product Information for SPRN elisa kit
Description: This Mouse Shadow of prion protein (SPRN) ELISA Kit employs a two-site sandwich ELISA to quantitate SPRN in samples. An antibody specific for SPRN has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SPRN present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SPRN is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SPRN bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: SPRN expression in human, rat, and mouse brain. The deduced human protein contains 151 amino acids. The mammalian proteins share 81 to 95% sequence identity. Alignment of all fish and mammalian Sho proteins showed that all have an N-terminal peptide sequence with an endoplasmic reticulum targeting signal for extracellular transport, a basic RG-rich region, a hydrophobic stretch in the middle of the protein that contains the same unusual composition of small aliphatic residues (GAV) as PrP and PrP-like proteins, and a C-terminal region with a putative N-glycosylation site and a possible GPI anchor site (Premzl et al., 2003, 2004). Database searches revealed that SPRN is expressed in mouse and rat embryo, brain, and retina, in human hippocampus, and in zebrafish embryo and retina.
Background: SPRN expression in human, rat, and mouse brain. The deduced human protein contains 151 amino acids. The mammalian proteins share 81 to 95% sequence identity. Alignment of all fish and mammalian Sho proteins showed that all have an N-terminal peptide sequence with an endoplasmic reticulum targeting signal for extracellular transport, a basic RG-rich region, a hydrophobic stretch in the middle of the protein that contains the same unusual composition of small aliphatic residues (GAV) as PrP and PrP-like proteins, and a C-terminal region with a putative N-glycosylation site and a possible GPI anchor site (Premzl et al., 2003, 2004). Database searches revealed that SPRN is expressed in mouse and rat embryo, brain, and retina, in human hippocampus, and in zebrafish embryo and retina.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
shadow of prion protein
NCBI Official Synonym Full Names
shadow of prion protein
NCBI Official Symbol
Sprn
NCBI Official Synonym Symbols
C630041J07
NCBI Protein Information
shadow of prion protein
UniProt Protein Name
Shadow of prion protein
Protein Family
UniProt Gene Name
Sprn
UniProt Synonym Gene Names
Gm169; Protein shadoo
UniProt Entry Name
SPRN_MOUSE
Uniprot Description
SPRN: Prion-like protein that has PrP(C)-like neuroprotective activity. May act as a modulator for the biological actions of normal and abnormal PrP. Belongs to the SPRN family.
Protein type: Membrane protein, GPI anchor
Cellular Component: membrane; cell; nucleolus; plasma membrane; intracellular; cytosol; nucleus; vesicle
Molecular Function: nucleic acid binding
Biological Process: protein import into nucleus
Research Articles on SPRN
Similar Products
Product Notes
The Mouse SPRN sprn (Catalog #AAA9716854) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9716854 ELISA Kit recognizes Mouse SPRN. It is sometimes possible for the material contained within the vial of "Shadow Of Prion Protein (SPRN), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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