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Western Blot (WB) (Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane: Hela cells, Primary antibody: 860017(ABCB9 Antibody) at dilution 1/250, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute)

Rabbit anti-Human PGP9 Polyclonal Antibody | anti-PGP9 antibody

PGP9 Rabbit pAb

Reactivity
Human
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Affinity purified
Synonyms
PGP9; Polyclonal Antibody; PGP9 Rabbit pAb; ATP-binding cassette; sub-family B (MDR/TAP); member 9; TAPL; EST122234; anti-PGP9 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Purity/Purification
Affinity purified
Form/Format
-20 degree C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Applicable Applications for anti-PGP9 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)
Application Notes
WB: 1:200-1:1000
IHC: 1:15-1:50
Immunogen
Synthetic peptide of human ABCB9
Conjugation
Unconjugated
Modification
Unmodified
Cell Position
Lysosome, Membrane
Preparation and Storage
Store at 4 degree C for short term. Store at -20 degree C for long term. Avoid freeze/thaw cycle.

Western Blot (WB)

(Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane: Hela cells, Primary antibody: 860017(ABCB9 Antibody) at dilution 1/250, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute)

Western Blot (WB) (Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane: Hela cells, Primary antibody: 860017(ABCB9 Antibody) at dilution 1/250, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute)

Immunohistochemistry (IHC)

(The image is immunohistochemistry of paraffin-embedded Human gasrtic cancer tissue using 860017(ABCB9 Antibody) at dilution 1/15, on the right is treated with synthetic peptide. (Original magnification: ×200))

Immunohistochemistry (IHC) (The image is immunohistochemistry of paraffin-embedded Human gasrtic cancer tissue using 860017(ABCB9 Antibody) at dilution 1/15, on the right is treated with synthetic peptide. (Original magnification: ×200))

Immunohistochemistry (IHC)

(The image is immunohistochemistry of paraffin-embedded Human breast cancer tissue using 860017(ABCB9 Antibody) at dilution 1/15, on the right is treated with synthetic peptide. (Original magnification: ×200))

Immunohistochemistry (IHC) (The image is immunohistochemistry of paraffin-embedded Human breast cancer tissue using 860017(ABCB9 Antibody) at dilution 1/15, on the right is treated with synthetic peptide. (Original magnification: ×200))
Product Categories/Family for anti-PGP9 antibody

NCBI and Uniprot Product Information

UniProt Accession #
Molecular Weight
84kDa
UniProt Protein Name
ATP-binding cassette sub-family B member 9
UniProt Gene Name
ABCB9
UniProt Synonym Gene Names
KIAA1520; ABC transporter 9 protein; hABCB9; TAPL
UniProt Entry Name
ABCB9_HUMAN

Uniprot Description

ABCB9: ATP-dependent low-affinity peptide transporter which translocates a broad spectrum of peptides from the cytosol to the lysosomal lumen. Displays a broad peptide length specificity from 6-mer up to at least 59-mer peptides with an optimum of 23-mers. Favors positively charged, aromatic or hydrophobic residues in the N- and C-terminal positions whereas negatively charged residues as well as asparagine and methionine are not favored. Belongs to the ABC transporter superfamily. ABCB family. MHC peptide exporter (TC 3.A.1.209) subfamily. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Transporter, ABC family; Membrane protein, multi-pass; Membrane protein, integral; Transporter

Chromosomal Location of Human Ortholog: 12q24

Cellular Component: lysosomal membrane; lysosome; endoplasmic reticulum; early endosome; integral to membrane; integral to endoplasmic reticulum membrane

Molecular Function: protein binding; protein homodimerization activity; substrate-specific transmembrane transporter activity; ATP binding; peptide-transporting ATPase activity

Biological Process: peptide transport; antigen processing and presentation of peptide antigen via MHC class I; protein transport; metabolic process; transmembrane transport

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Product Notes

The PGP9 abcb9 (Catalog #AAA4758231) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PGP9 Rabbit pAb reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PGP9 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P). WB: 1:200-1:1000 IHC: 1:15-1:50. Researchers should empirically determine the suitability of the PGP9 abcb9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PGP9, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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