Low Affinity Immunoglobulin Gamma Fc Region Receptor IV Active Protein | Fcgr4 active protein

Recombinant Mouse Low Affinity Immunoglobulin Gamma Fc Region Receptor IV Protein

Cat. Num. AAA2556799

• Gene Names: Fcgr4; Fcrl3; CD16-2; FcgRIV; Fcgr3a; FcgammaRIV; 4833442P21Rik
• Purity: >95% as determined by reducing SDS-PAGE.
• Synonyms: Low Affinity Immunoglobulin Gamma Fc Region Receptor IV; Recombinant Mouse Low Affinity Immunoglobulin Gamma Fc Region Receptor IV Protein; FcgR4; CD16-2; Fcgr4 active protein

• Host: Human Cells
• Purity/Purification: >95% as determined by reducing SDS-PAGE.
• Form/Format: Lyophilized from a 0.2 mum filtered solution of PBS, pH 7.4.
• Sequence Length: 249

• Species: Mouse
• Endotoxin: <1.0 EU per mug as determined by LAL test.
• Fragment: Met 1-Gln 203
• Fusion Tag: C-6His
• Activity: 1. Measured by its ability to bind human IgG1 (Fc) in a functional ELISA. 2. Labeling ratio of biotin to protein: 0.6
• Preparation and Storage: Lyophilized protein should be stored at < -20 degree C, though stable at room temperature for 3 weeks.; Reconstituted protein solution can be stored at 4-7 degree C for 2-7 days.; Aliquots of reconstituted samples are stable at < -20 degree C for 3 months.

Related Product Information for Fcgr4 active protein

Protein Construction: Recombinant Mouse Low Affinity Immunoglobulin Gamma Fc Region Receptor IV is produced by our Mammalian expression system and the target gene encoding Gly21-Gln203 is expressed with a 6His tag at the C-terminus.

Background: Fcgr4, also known as CD16-2, is one of the receptors for Fc region of IgG which involve in immune responses. Fcgr4 mainly functions in cellular response to lipopolysaccharide, NK T cell proliferation, regulation of sensory perception of pain, wound healing etc. Three groups are included for Fc gamma receptors (FcR), and they are Fc gamma RI (CD64), Fc gamma RII (CD32), and Fc gamma RIII (CD16). Among these, CD64 possess high affinity even for monomeric IgG, while CD32 and CD16 display a relative lower affinity for IgG. Genes encodes these receptors are diverse differing by species and cell types. The aggregation of FcR having immunoreceptor tyrosine-based activation motifs (ITAMs) activates sequentially src family tyrosine kinases and syk family tyrosine kinases that connect transduced signals to common activation pathways shared with other receptors. FcR with ITAMs elicit cell activation, endocytosis, and phagocytosis. Fcgr4 belongs to Fc gamma RIII (CD16) group.

NCBI and Uniprot Product Information

• NCBI GI #: 224922770
• NCBI GeneID: 246256
• NCBI Accession #: NP_653142.2
• NCBI GenBank Nucleotide #: NM_144559.2
• UniProt Accession #: A0A0B4J1G0; Q3TC44; Q8R2R4; Q8R477
• Molecular Weight: Molecular Mass: 21.9kDa; Actual Protein Molecular Mass: 28-35kDa
• NCBI Official Full Name: low affinity immunoglobulin gamma Fc region receptor IV
• NCBI Official Synonym Full Names: Fc receptor, IgG, low affinity IV
• NCBI Official Symbol: Fcgr4
• NCBI Official Synonym Symbols: Fcrl3; CD16-2; FcgRIV; Fcgr3a; FcgammaRIV; 4833442P21Rik
• NCBI Protein Information: low affinity immunoglobulin gamma Fc region receptor IV
• UniProt Protein Name: Low affinity immunoglobulin gamma Fc region receptor IV
• UniProt Gene Name: Fcgr4

Uniprot Description

Receptor for the Fc region of immunoglobulin gamma (PubMed:16039578). Also acts as a receptor for the Fc region of immunoglobulin epsilon (PubMed:17558411, PubMed:18949059). Binds with intermediate affinity to both IgG2a and IgG2b (PubMed:16039578, PubMed:17558411, PubMed:19795417). Can bind to IgG2a and IgG2b monomers (PubMed:18949059). Does not display binding to IgG1 or IgG3 (PubMed:16039578). Mediates neutrophil activation by IgG complexes redundantly with Fcgr3 (PubMed:18097064). Plays a role in promoting bone resorption by enhancing osteoclast differentiation following binding to IgG2a (PubMed:25824719). Binds with low affinity to both the a and b allotypes of IgE (PubMed:18949059). Has also been shown to bind to IgE allotype a only but not to allotype b (PubMed:17558411). Binds aggregated IgE but not the monomeric form and bound monomeric IgG is readily displaced by IgE complexes (PubMed:18949059). Binding to IgE promotes macrophage-mediated phagocytosis, antigen presentation to T cells, production of proinflammatory cytokines and the late phase of cutaneous allergic reactions (PubMed:17558411, PubMed:18949059).