Fc gamma RIIB/CD32b Active Protein | FCGR2B active protein

Human CellExp Fc gamma RIIB / CD32b, human recombinant

Cat. Num. AAA844984

• Gene Names: FCGR2B; CD32; FCG2; CD32B; FCGR2; IGFR2
• Applications: SDS-Page
• Purity: >=95%
• Synonyms: Fc gamma RIIB/CD32b; Human CellExp Fc gamma RIIB / CD32b; human recombinant; FCGR2B; CD32b; CD32; FCG2; IGFR2; CDw32; FCGR2B active protein

• Host: HEK293 cells
• Purity/Purification: >=95%
• Form/Format: Lyophilized from 0.22 mum filtered solution in PBS, pH7.4. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.; Appearance: Lyophilized
• Sequence Length: 290

• Applicable Applications for FCGR2B active protein: SDS-PAGE
• Solubility/Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 ug/ml. Do not vortex. This solution can be stored at 2-8 degree C for up to 1 month. For extended storage, it is recommended to store at -20 degree C.
• Endotoxin Level: <1 EU/mug by LAL method
• Biological Activity: Measured by its ability to bind human IgG with an estimated Kd <150 nM.
• Results: Measured by its ability to bind human IgG with an estimated Kd <150 nM.
• Handling: Centrifuge the vial prior to opening.
• Preparation and Storage: At -20 degree C; Shelf Life: 12 months

Testing Data

Related Product Information for FCGR2B active protein

Background: Receptors for the Fc region of IgG (Fc gamma R) are members of the Ig superfamily that function in the activation or inhibition of immune responses. Three classes of human Fc gamma Rs: RI (CD64), RII (CD32), and RIII (CD16), which generate multiple isoforms, are recognized. There are three genes for human Fcgamma RII /CD32 (A, B, and C) and one for mouse Fcgamma RII B (CD32B). CD32 is a low affinity receptor for IgG. Low affinity immunoglobulin gamma Fc region receptor II-b (FCGR2B) also known as CD32b, FCG2, IGFR2. CD32B is expressed on B cells and myeloid dendritic cells. Ligation of CD32B on B cells down regulates antibody production and may, in some circumstances, promote apoptosis. Co-ligation of CD32B on dendritic cells inhibits maturation and blocks cell activation. CD32B may also be a target for monoclonal antibody therapy for malignancies.

Product Categories/Family for FCGR2B active protein

Proteins and Enzymes; Human cell expressed recombinant proteinsProteins and Enzymes; Cytokines; Growth Factors & Hormones; CDsProteins and Enzymes; Cytokines; Growth Factors & Hormones; Cytokines & Growth Factors (A-Z) Proteins and Enzymes; Proteins and E

NCBI and Uniprot Product Information

• NCBI GI #: 50511932
• NCBI GeneID: 2213
• NCBI Accession #: NP_001002273.1
• NCBI GenBank Nucleotide #: NM_001002273.2
• UniProt Accession #: P31994; O95649; Q53X85; Q5VXA9; Q8NIA1; A6H8N3
• Molecular Weight: This protein is fused with 6×His tag at the C-terminus, has a calculated MW of 20.2 kDa. The predicted N-terminus is Ala 46. DTT-reduced Protein migrates as 23-33 kDa due to different glycosylation.
• NCBI Official Full Name: low affinity immunoglobulin gamma Fc region receptor II-b isoform 2
• NCBI Official Synonym Full Names: Fc fragment of IgG, low affinity IIb, receptor (CD32)
• NCBI Official Symbol: FCGR2B
• NCBI Official Synonym Symbols: CD32; FCG2; CD32B; FCGR2; IGFR2
• NCBI Protein Information: low affinity immunoglobulin gamma Fc region receptor II-b
• UniProt Protein Name: Low affinity immunoglobulin gamma Fc region receptor II-b
• Protein Family: Low affinity immunoglobulin gamma Fc region receptor
• UniProt Gene Name: FCGR2B
• UniProt Synonym Gene Names: CD32; FCG2; IGFR2; IgG Fc receptor II-b; Fc-gamma-RIIb; FcRII-b
• UniProt Entry Name: FCG2B_HUMAN

NCBI Description

The protein encoded by this gene is a low affinity receptor for the Fc region of immunoglobulin gamma complexes. The encoded protein is involved in the phagocytosis of immune complexes and in the regulation of antibody production by B-cells. Variations in this gene may increase susceptibilty to systemic lupus erythematosus (SLE). Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2010]

Uniprot Description

FCGR2B: a transmembrane receptor for the Fc region of complexed or aggregated immunoglobulin gamma (IgG). Involved in a variety of effector and regulatory functions such as phagocytosis of immune complexes and modulation of antibody production by B-cells. Binding to this receptor results in down modulation of previous state of cell activation triggered via antigen receptors on B cells, T cells or via another Fc receptor. Three splice-variant isoforms have been observed.

Protein type: Oncoprotein; Membrane protein, integral; Cell surface

Chromosomal Location of Human Ortholog: 1q23

Cellular Component: integral to membrane; plasma membrane

Molecular Function: protein binding; IgG binding

Biological Process: regulation of immune response; viral reproduction; immune response; signal transduction

Disease: Systemic Lupus Erythematosus; Malaria, Susceptibility To