Interleukin-12 Receptor Subunit beta-1 Active Protein | IL12RB1 active protein

Recombinant Human Interleukin-12 Receptor Subunit beta-1 Protein

Cat. Num. AAA2556658

• Gene Names: IL12RB1; CD212; IMD30; IL12RB; IL-12R-BETA1
• Purity: >95% as determined by reducing SDS-PAGE.
• Synonyms: Interleukin-12 Receptor Subunit beta-1; Recombinant Human Interleukin-12 Receptor Subunit beta-1 Protein; CD212; IL12RB1; CD212 antigen; IL-12 receptor beta component; IL-12 receptor subunit beta-1; IL12R; IL-12R subunit beta-1; IL12RB; IL-12RB1; IL-12R-BETA1; IL-12R-beta-1; interleukin-12 receptor beta-1 chain; interleukin-12 receptor subunit beta-1; IL12RB1 active protein

• Host: Human Cells
• Purity/Purification: >95% as determined by reducing SDS-PAGE.
• Form/Format: Lyophilized from a 0.2 mum filtered solution of PBS, pH 7.4.
• Sequence Length: 662

• Species: Human
• Endotoxin: <1.0 EU per ug as determined by LAL test.
• Fragment: Cys24-Glu540
• Fusion Tag: C-Fc
• Activity: Measured by its binding ability in a functional ELISA. Immobilized human IL12RB1 at 20 mug/ml (100 mul/well) can bind human IL12B with a linear ranger of 2.56-64 ng/ml.
• Preparation and Storage: Lyophilized protein should be stored at < -20 degree C, though stable at room temperature for 3 weeks.; Reconstituted protein solution can be stored at 4-7 degree C for 2-7 days.; Aliquots of reconstituted samples are stable at < -20 degree C for 3 months.

Related Product Information for IL12RB1 active protein

Protein Construction: Recombinant Human Interleukin-12 receptor subunit beta-1 is produced by our Mammalian expression system and the target gene encoding Cys24-Glu540 is expressed with a Fc tag at the C-terminus.

Background: Interleukin12 receptor subunit beta 1 (IL12RB1) is a type I transmembrane protein that belongs to the hemopoietin receptor superfamily. IL12RB1 can spontaneously form homodimers and -oligomers, which are able to bind IL12 with only low affinity. IL12 high affinity receptor complex is composed of two subunits designated IL12RB1 and IL12RB2. While IL12RB1 interacts with the IL-12p40 subunit, IL-12p35 is mainly connecting with IL12RB2. This receptor chain is also responsible for transmitting the IL12 signal into the cell. IL12RB1, to the contrary, is also part of the IL23R, where it interacts with the p40 subunit of IL23. IL12RB1 is expressed in activated T cells, NK cells and B cells.

NCBI and Uniprot Product Information

• NCBI GI #: 5031785
• NCBI GeneID: 3594
• NCBI Accession #: NP_005526.1
• NCBI GenBank Nucleotide #: NM_005535.2
• UniProt Accession #: P42701; Q8N6Q7; A8K308; B2RPF1; B7ZKK3
• Molecular Weight: Molecular Mass: 84.2kDa; Actual Protein Molecular Mass: 100-110kDa
• NCBI Official Full Name: interleukin-12 receptor subunit beta-1 isoform 1
• NCBI Official Synonym Full Names: interleukin 12 receptor subunit beta 1
• NCBI Official Symbol: IL12RB1
• NCBI Official Synonym Symbols: CD212; IMD30; IL12RB; IL-12R-BETA1
• NCBI Protein Information: interleukin-12 receptor subunit beta-1
• UniProt Protein Name: Interleukin-12 receptor subunit beta-1
• Protein Family: Interleukin-12 receptor
• UniProt Gene Name: IL12RB1
• UniProt Synonym Gene Names: IL12R; IL12RB; IL-12 receptor subunit beta-1; IL-12R subunit beta-1; IL-12R-beta-1; IL-12RB1

NCBI Description

The protein encoded by this gene is a type I transmembrane protein that belongs to the hemopoietin receptor superfamily. This protein binds to interleukine 12 (IL12) with a low affinity, and is thought to be a part of IL12 receptor complex. This protein forms a disulfide-linked oligomer, which is required for its IL12 binding activity. The coexpression of this and IL12RB2 proteins was shown to lead to the formation of high-affinity IL12 binding sites and reconstitution of IL12 dependent signaling. Mutations in this gene impair the development of interleukin-17-producing T lymphocytes and result in increased susceptibility to mycobacterial and Salmonella infections. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014]

Uniprot Description

Functions as an interleukin receptor which binds interleukin-12 with low affinity and is involved in IL12 transduction. Associated with IL12RB2 it forms a functional, high affinity receptor for IL12. Associates also with IL23R to form the interleukin-23 receptor which functions in IL23 signal transduction probably through activation of the Jak-Stat signaling cascade.