GDNF active protein

Recombinant Human GDNF

Cat. Num. AAA4159727

• Gene Names: GDNF; ATF; ATF1; ATF2; HSCR3; HFB1-GDNF
• Purity: >95% as determined by SDS-PAGE
• Synonyms: GDNF; Recombinant Human GDNF; Glial cell line-derived neurotrophic factor; Astrocyte-derived trophic factor; ATF; Recombinant Human Glial-Derived Neurotrophic Factor (GDNF); GDNF active protein

• Host: E Coli
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from a 0.2 um filtered solution in Sodium Citrate and NaCl (pH 4.0)
• Sequence Length: 159

• Bioactivity: The ED50 as determined by the proliferation of rat C6 cells was found to be <0.1 ng/mL
• Endotoxin Level: <1.0 EU/ug of recombinant protein as determined by the LAL method
• Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
• Preparation and Storage: The lyophilized protein is stable for at least one year from date of receipt at-70 degree C. Upon reconstitution, this cytokine can be stored in working aliquots at 2-8 degree C for one month, or at-20 degree C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

Testing Data

Testing Data

Related Product Information for GDNF active protein

GDNF is a disulfide-linked, homodimeric neurotrophic factor that belongs to the cystine-knot family of growth factors. Natively glycosylated, GDNF signals through a multi-component receptor system that comprises of a RET and one of the four GFRalpha receptors. In embryonic midbrain cultures, GDNF promotes the survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake. Mature rat and human GDNF exhibit approximately 93% amino acid sequence identity and show considerable species cross-reactivity Recombinant Human GDNF is a non-glycosylated, disulfide-linked homodimer (30 kDa) consisting of 135 amino acids in each subunit.

NCBI and Uniprot Product Information

• NCBI GI #: 493796159
• NCBI GeneID: 2668
• NCBI Accession #: NP_001265027.1
• NCBI GenBank Nucleotide #: NP_001265027.1
• UniProt Accession #: P39905
• NCBI Official Full Name: glial cell line-derived neurotrophic factor isoform 5
• NCBI Official Synonym Full Names: glial cell derived neurotrophic factor
• NCBI Official Symbol: GDNF
• NCBI Official Synonym Symbols: ATF; ATF1; ATF2; HSCR3; HFB1-GDNF
• NCBI Protein Information: glial cell line-derived neurotrophic factor
• UniProt Protein Name: Glial cell line-derived neurotrophic factor
• Protein Family: GDNF family receptor
• UniProt Gene Name: GDNF
• UniProt Synonym Gene Names: hGDNF; ATF
• UniProt Entry Name: GDNF_HUMAN

NCBI Description

This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. The recombinant form of this protein, a highly conserved neurotrophic factor, was shown to promote the survival and differentiation of dopaminergic neurons in culture, and was able to prevent apoptosis of motor neurons induced by axotomy. This protein is a ligand for the product of the RET (rearranged during transfection) protooncogene. Mutations in this gene may be associated with Hirschsprung disease and Tourette syndrome. This gene encodes multiple protein isoforms that may undergo similar proteolytic processing. [provided by RefSeq, Aug 2016]

Uniprot Description

GDNF: Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake. Defects in GDNF may be a cause of Hirschsprung disease type 3 (HSCR3). In association with mutations of RET gene, defects in GDNF may be involved in Hirschsprung disease. This genetic disorder of neural crest development is characterized by the absence of intramural ganglion cells in the hindgut, often resulting in intestinal obstruction. Defects in GDNF are a cause of congenital central hypoventilation syndrome (CCHS); also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. Belongs to the TGF-beta family. GDNF subfamily. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 5p13.1-p12

Cellular Component: extracellular region

Molecular Function: protein homodimerization activity; growth factor activity; receptor binding

Biological Process: nervous system development; axon guidance; positive regulation of dopamine secretion; peristalsis; adult locomotory behavior; mRNA stabilization; positive regulation of monooxygenase activity; regulation of dopamine uptake; signal transduction; enteric nervous system development; sympathetic nervous system development; regulation of gene expression; ureteric bud branching; induction of an organ; positive regulation of cell proliferation; positive regulation of transcription from RNA polymerase II promoter; postganglionic parasympathetic nervous system development; negative regulation of neuron apoptosis; postsynaptic membrane organization; positive regulation of cell differentiation; neural crest cell migration; metanephros development; neurite development; negative regulation of apoptosis

Disease: Hirschsprung Disease, Susceptibility To, 3; Central Hypoventilation Syndrome, Congenital; Pheochromocytoma