Testing Data
Human Oct4 Transcription Factor Assay Kit
Oct4 (Human) Transcription Factor Activity Assay Kit
Reactivity
Human
Synonyms
Oct4 Transcription Factor; Oct4 (Human) Transcription Factor Activity Assay Kit; Oct4 Transcription Factor assay kit
Reactivity
Human
Samples
Nuclear extraction, whole lysates
Detection Method
Absorbance: 450nm
Features & Benefits
A non-radioactive transcription factor assay with an ELISA format to detect the activation of Oct4.
An easy, speedy, sensitive and high-throughput method to detect the activation of transcription factors.
An easy, speedy, sensitive and high-throughput method to detect the activation of transcription factors.
Preparation and Storage
Store at -20 degree C
Shelf life: ~12 months
Shelf life: ~12 months
Testing Data
Testing Data
Testing Data
Testing Data
Related Product Information for Oct4 Transcription Factor assay kit
Pluripotency and differentiation of embryonic stem (ES) cells is controlled by regulatory genes, which are often transcriptional regulators. The roles of these regulators are to repress or activate patterns of gene expression for normal pluripotent cell development and maintenance, and to mediate permanent phenotypic change during stem cell differentiation. Oct4 is one of these important transcription factors specifically expressed in cells with pluripotent identity. For example, a study with Oct4-null mouse ES cells that differentiated into trophectoderm, the lineage that supplies trophoblast cells for the developing placenta, defined the effects of Oct4 on ES cell self-renewal1. Oct4 belongs to the Octamer class of transcription factors because of recognition of an 8-bp consensus ATGCAAAT DNA sequence. Together with Pit and Unc proteins, Oct proteins define the POU (Pit, Oct and Unc) domain that interact with DNA by binding to the 8-bp sequence. Oct4 contains two DNA-binding domains: the 75-amino-acid POU-specific domain (POUS) and the 60-amino-acid carboxy-terminal POU homeodomain (POUHD). Accurate monitoring of the level of activated Oct4 in cells, tissues or animal models is required for investigating signal transduction pathways and other research applications such as drug development. Simple, speedy and high-throughput methods are required for this purpose. The Oct4 Transcription Factor-Activity Assay kit is a non-radioactive transcription factor assay with an ELISA format. It offers an easy, speedy, sensitive and high-throughput method to detect the activation of transcription factors.
Product Categories/Family for Oct4 Transcription Factor assay kit
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Product Notes
The Human Oct4 Transcription Factor (Catalog #AAA849117) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA849117 Assay Kit recognizes Human Oct4 Transcription Factor. It is sometimes possible for the material contained within the vial of "Oct4 Transcription Factor, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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