<
Immunofluorescence (IF)
(MBS9604892 staining COLO205 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
Rabbit SLK Polyclonal Antibody | anti-SLK antibody
SLK Antibody
Gene Names
SLK; LOSK; STK2; se20-9; bA16H23.1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, ELISA
Purity
Peptide affinity purification
Synonyms
SLK; Polyclonal Antibody; SLK Antibody; CTCL tumor antigen se20 9; LOSK; se20 9; Serine/threonine protein kinase 2; SNF1 sucrose nonfermenting like kinase; STE20 like serine/threonine-protein kinase; STE20 related kinase; STK2; anti-SLK antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
SLK antibody detects endogenous levels of total SLK
Purity/Purification
Peptide affinity purification
Form/Format
Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
1204
Applicable Applications for anti-SLK antibody
Western Blot (WB), Immunohistochemisty (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes
WB: 1:500-1:1000
IHC: 1:50-1:200
IF/ICC: 1:100-1:500
EIA: 1:20000-1:40000
IHC: 1:50-1:200
IF/ICC: 1:100-1:500
EIA: 1:20000-1:40000
Immunogen
A synthesized peptide derived from human SLK, corresponding to a region within C-Terminal amino acids.
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Immunofluorescence (IF)
(MBS9604892 staining COLO205 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
Immunofluorescence (IF)
(MBS9604892 staining COLO205 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
Western Blot (WB)
(Western blot analysis of extracts from rat lung, using SLK Antibody.)
Western Blot (WB)
(Western blot analysis of extracts from rat lung, using SLK Antibody.)
Western Blot (WB)
(Western blot analysis of extracts from COLO205 cells, using SLK antibody.)
Western Blot (WB)
(Western blot analysis of extracts from COLO205 cells, using SLK antibody.)
Immunohistochemistry (IHC)
(MBS9604892 at 1/100 staining Human kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunohistochemistry (IHC)
(MBS9604892 at 1/100 staining Human kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Related Product Information for anti-SLK antibody
Function: Mediates apoptosis and actin stress fiber dissolution.
Post-translational Modifications: Proteolytically cleaved by caspase-3. Autophosphorylated.
Similarity: Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
Post-translational Modifications: Proteolytically cleaved by caspase-3. Autophosphorylated.
Similarity: Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
150kDa
NCBI Official Full Name
STE20-like serine/threonine-protein kinase isoform 2
NCBI Official Synonym Full Names
STE20 like kinase
NCBI Official Symbol
SLK
NCBI Official Synonym Symbols
LOSK; STK2; se20-9; bA16H23.1
NCBI Protein Information
STE20-like serine/threonine-protein kinase
UniProt Protein Name
STE20-like serine/threonine-protein kinase
Protein Family
UniProt Gene Name
SLK
UniProt Synonym Gene Names
KIAA0204; STK2; STE20-like kinase; hSLK; STE20-related kinase
Uniprot Description
Mediates apoptosis and actin stress fiber dissolution.
Research Articles on SLK
Similar Products
Product Notes
The SLK slk (Catalog #AAA9604892) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SLK Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's SLK can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemisty (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA). WB: 1:500-1:1000 IHC: 1:50-1:200 IF/ICC: 1:100-1:500 EIA: 1:20000-1:40000. Researchers should empirically determine the suitability of the SLK slk for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SLK, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
