Western Blot (WB)
(Anti-PI 3 Kinase p85 alpha antibody, MBS175626, Western blottingLane 1: MCF-7 Cell LysateLane 2: HELA Cell LysateLane 3: COLO Cell LysateLane 4: SW620 Cell Lysate )
Rabbit PI 3 Kinase p85 alpha Polyclonal Antibody | anti-PIK3R1 antibody
Anti-PI 3 Kinase p85 alpha antibody
Western Blot (WB)
(Anti-PI 3 Kinase p85 alpha antibody, MBS175626, Western blottingLane 1: MCF-7 Cell LysateLane 2: HELA Cell LysateLane 3: COLO Cell LysateLane 4: SW620 Cell Lysate )
Western Blot (WB)
(Anti-PI 3 Kinase p85 alpha antibody, MBS175626, Western blottingLane 1: MCF-7 Cell LysateLane 2: HELA Cell LysateLane 3: COLO Cell LysateLane 4: SW620 Cell Lysate )
Background: Phosphatidylinositol 3-kinase regulatory subunit alpha is an enzyme that in humans is encoded by the PIK3R1 gene. Its gene is mapped to 5q13. the bovine PI3K p85 subunit consists of 2 closely related proteins, p85-alpha and p85-beta. They cloned cDNAs encoding both p85 subunits, each of which is a 724-amino acid polypeptide. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance.
NCBI and Uniprot Product Information
NCBI Description
Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in four transcript variants encoding different isoforms. [provided by RefSeq, Jun 2011]
Uniprot Description
PIK3R1: regulatory subunit of phosphoinositide-3-kinase. Mediates binding to a subset of tyrosine-phosphorylated proteins through its SH2 domain. Acts as an adapter, mediating the association of the p110 catalytic unit of the alpha, beta and delta enzymes to the plasma membrane, where p110 phosphorylates inositol lipids. May play an additional role in the regulation of the actin cytoskeleton. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. Its SH2 domains interacts with the YTHM motif of phosphorylated INSR in vitro. Defects in PIK3R1 are a cause of severe insulin resistance. Four alternatively spliced isoforms have been described.
Protein type: Enzyme, regulatory subunit; Kinase, lipid; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 5q13.1
Cellular Component: membrane; cytoplasm; plasma membrane; intercellular junction; phosphoinositide 3-kinase complex; cytosol; nucleus
Molecular Function: insulin binding; insulin-like growth factor receptor binding; protein binding; ErbB-3 class receptor binding; insulin receptor substrate binding; neurotrophin TRKA receptor binding; phosphoinositide 3-kinase binding; phosphoinositide 3-kinase regulator activity; transmembrane receptor protein tyrosine kinase adaptor protein activity; protein phosphatase binding; transcription factor binding; insulin receptor binding
Biological Process: negative regulation of osteoclast differentiation; phosphoinositide 3-kinase cascade; viral reproduction; nerve growth factor receptor signaling pathway; positive regulation of RNA splicing; regulation of phosphoinositide 3-kinase activity; protein amino acid phosphorylation; NFAT protein import into nucleus; T cell receptor signaling pathway; positive regulation of glucose import; phosphoinositide phosphorylation; phospholipid metabolic process; positive regulation of transcription factor import into nucleus; epidermal growth factor receptor signaling pathway; platelet activation; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; protein stabilization; negative regulation of cell-matrix adhesion; positive regulation of tumor necrosis factor production; induction of apoptosis via death domain receptors; insulin-like growth factor receptor signaling pathway; cellular response to insulin stimulus; DNA damage response, signal transduction resulting in induction of apoptosis; B cell differentiation; T cell costimulation; phosphatidylinositol biosynthetic process; insulin receptor signaling pathway; innate immune response; cell glucose homeostasis; positive regulation of transcription from RNA polymerase II promoter; blood coagulation; vascular endothelial growth factor receptor signaling pathway; leukocyte migration; positive regulation of cell migration; negative regulation of apoptosis
Disease: Agammaglobulinemia 7, Autosomal Recessive; Short Syndrome; Immunodeficiency 36
Research Articles on PIK3R1
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Product Notes
The PIK3R1 pik3r1 (Catalog #AAA175626) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-PI 3 Kinase p85 alpha antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PI 3 Kinase p85 alpha can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the PIK3R1 pik3r1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PI 3 Kinase p85 alpha, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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