Interleukin-13 ELISA Kit | IL-13 Ra2 elisa kit

Interleukin-13 Receptor Alpha 2 (IL-13 Ra2)

Cat. Num. AAA590018

• Gene Names: IL13; P600; IL-13
• Synonyms: Interleukin-13; Interleukin-13 Receptor Alpha 2 (IL-13 Ra2); IL-13 Ra2 elisa kit

• Specificity: This sandwich ELISA recognises both natural and recombinant human IL-13 Ra2. This kit has been tested and exhibited no significant cross-reactivity with following cytokines and growth factors: IL-1alpha, IL-1beta, IL-2, IL-4, IL-5, Il-6, IL-7, IL-8, IL-10, IL- 11, Il-12, Il-17A, FGF basic, GM-CSF, IFN-gamma, M-CSF, MCP-1(MCAF), MCP3, EGF, TNF- alpha, TNF-beta
• Sequence Length: 146

• Samples: Cell Culture Supernatant
• Assay Type: Sandwich
• Intended Uses: This Human Interleukin 13 Ralpha2 ELISA Kit is to be used for the in vitro quantitative determination of human interleukin 13 Ralpha2 concentrations in cell culture supernatant. This kit is intended FOR LABORATORY RESEARCH USE ONLY

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IL-13 Ra2 elisa kit

Principle of the assay: This assay applies the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific to IL-13 Ralpha2 has been pre-coated onto a microplate. Standards and samples are then added to the appropriate plate wells with a biotin - conjugated antibody preparation specific for IL-13 Ralpha2 and incubated. IL-13 Ralpha2 if present, will bind and become immobilized by the antibody pre-coated on the wells and then be "sandwiched" by biotin conjugate. Avidin is a tetramer containing four identical subunits that each has a high affinity-binding site for biotin. After washing away any unbound substances, avidin-Horseradish Peroxidase (HRP) will be added to each well and incubated. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and only those wells that contain IL-13 Ralpha2, biotin conjugated antibody and enzyme-conjugated Avidin will exhibit a change in colour. The colour development is stopped and the intensity of the colour is measured. In order to measure the concentration of IL-13 Ralpha2 in the samples, this package of reagent includes two calibration diluents (Calibrator Diluent I for serum/plasma testing and Calibrator Diluent II for cell culture supernatant testing.) According to the testing system, the provided standard is diluted (2-fold) with the appropriate Calibrator Diluent and assayed at the same time as the samples. This allows the operator to produce a standard curve of Optical Density (O.D) versus IL-13 Ralpha2 concentration. The concentration of IL-13 Ralpha2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for IL-13 Ra2 elisa kit

ELISA Kits for Human Cytokines and Receptors

NCBI and Uniprot Product Information

• NCBI GI #: 26787978
• NCBI GeneID: 3596
• NCBI Accession #: NP_002179.2
• NCBI GenBank Nucleotide #: NM_002188.2
• Molecular Weight: 15,816 Da
• NCBI Official Full Name: interleukin-13
• NCBI Official Synonym Full Names: interleukin 13
• NCBI Official Symbol: IL13
• NCBI Official Synonym Symbols: P600; IL-13
• NCBI Protein Information: interleukin-13
• UniProt Protein Name: Interleukin-13
• Protein Family: Interleukin
• UniProt Gene Name: IL13
• UniProt Entry Name: IL13_HUMAN

NCBI Description

This gene encodes an immunoregulatory cytokine produced primarily by activated Th2 cells. This cytokine is involved in several stages of B-cell maturation and differentiation. It up-regulates CD23 and MHC class II expression, and promotes IgE isotype switching of B cells. This cytokine down-regulates macrophage activity, thereby inhibits the production of pro-inflammatory cytokines and chemokines. This cytokine is found to be critical to the pathogenesis of allergen-induced asthma but operates through mechanisms independent of IgE and eosinophils. This gene, IL3, IL5, IL4, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL4. [provided by RefSeq, Jul 2008]

Uniprot Description

IL13: Cytokine. Inhibits inflammatory cytokine production. Synergizes with IL2 in regulating interferon-gamma synthesis. May be critical in regulating inflammatory and immune responses. Defects in IL13 may be a cause of susceptibility to allergic rhinitis (ALRH). Allergic rhinitis is a common disease of complex inheritance and is characterized by mucosal inflammation caused by allergen exposure. Belongs to the IL-4/IL-13 family.

Protein type: Secreted; Motility/polarity/chemotaxis; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 5q31

Cellular Component: extracellular space; cytoplasm; extracellular region; external side of plasma membrane

Molecular Function: protein binding; interleukin-13 receptor binding; cytokine activity

Biological Process: response to nicotine; positive regulation of smooth muscle cell proliferation; microglial cell activation; regulation of proton transport; response to lipopolysaccharide; signal transduction; positive regulation of connective tissue growth factor production; positive regulation of tyrosine phosphorylation of Stat6 protein; positive regulation of immunoglobulin production; response to ethanol; cell-cell signaling; positive regulation of protein secretion; positive regulation of B cell proliferation; immune response; positive regulation of release of sequestered calcium ion into cytosol; cell motility; inflammatory response; negative regulation of NAD(P)H oxidase activity; positive regulation of macrophage activation

Disease: Asthma, Susceptibility To; Allergic Rhinitis