Human Histatin-3 ELISA Kit | HTN3 elisa kit

Human Histatin-3 ELISA Kit

Cat. Num. AAA765609

• Gene Names: HTN3; HIS2; HTN2; HTN5
• Reactivity: Human
• Synonyms: Histatin-3; Human Histatin-3 ELISA Kit; HTN3/Histatin-3/PB/Basic histidine-rich protein/Hst/Histidine-rich protein 3; HTN3 elisa kit

• Reactivity: Human
• Sequence Length: 51

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 12.5-800ng/ml
• Sensitivity: 7.5ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for HTN3 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human HTN3 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human HTN3 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human HTN3 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human HTN3 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 4557653
• NCBI GeneID: 3347
• NCBI Accession #: NP_000191.1
• NCBI GenBank Nucleotide #: NM_000200.2
• Molecular Weight: 6,149 Da
• NCBI Official Full Name: histatin-3
• NCBI Official Synonym Full Names: histatin 3
• NCBI Official Symbol: HTN3
• NCBI Official Synonym Symbols: HIS2; HTN2; HTN5
• NCBI Protein Information: histatin-3
• UniProt Protein Name: Histatin-3
• Protein Family: Histatin
• UniProt Gene Name: HTN3
• UniProt Synonym Gene Names: HIS2; Hst; His3 20/44; His3 1/25; His3 20/43; His3 1/24; His3 20/32; His3 1/13; His3 20/31; His3 1/12; His3 20/30; His3 1/11; His3 24/32; His3 5/13; His3 24/31; His3 5/12; His3 24/30; His3 5/11; His3 25/32; His3 6/13; His3 25/30; His3 6/11; His3 26/32; Hi
• UniProt Entry Name: HIS3_HUMAN

NCBI Description

This gene encodes a member of the histatin family of small, histidine-rich, cationic proteins. They function as antimicrobial peptides and are important components of the innate immune system. Histatins are found in saliva and exhibit antibacterial, antifungal activities and function in wound healing. [provided by RefSeq, Sep 2014]

Uniprot Description

HTN3: Histatins are salivary proteins that are considered to be major precursors of the protective proteinaceous structure on tooth surfaces (enamel pellicle). In addition, histatins exhibit antibacterial and antifungal activities. His3-(20-43)-peptide (histatin-5) is especially effective against C.albicans and C.neoformans, and inhibits Lys-gingipain and Arg-gingipain (rgpB) from P.gingivalis. In addition, His3-(20-43)-peptide is a potent inhibitor of metalloproteinases MMP2 and MMP9. Belongs to the histatin/statherin family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 4q13

Cellular Component: extracellular region

Molecular Function: metal ion binding; protein binding

Biological Process: biomineral formation; defense response to bacterium; defense response to fungus; killing of cells of another organism