Rabbit anti-Mouse TLX1 Polyclonal Antibody | anti-TLX1 antibody

TLX1, ID (TLX1, HOX11, TCL3, T-cell leukemia homeobox protein 1, Homeobox protein Hox-11, Proto-oncogene TCL-3, T-cell leukemia/lymphoma protein 3) (Biotin)

Cat. Num. AAA6355945

• Gene Names: TLX1; TCL3; HOX11
• Reactivity: Mouse
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: TLX1; Polyclonal Antibody; ID (TLX1; HOX11; TCL3; T-cell leukemia homeobox protein 1; Homeobox protein Hox-11; Proto-oncogene TCL-3; T-cell leukemia/lymphoma protein 3) (Biotin); T-cell leukemia/lymphoma protein 3; anti-TLX1 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-TLX1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: TLX1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 162-191 amino acids from the Central region of human TLX1.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(TLX1 Antibody (Center) western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the TLX1 antibody detected the TLX1 protein (arrow).)

Related Product Information for anti-TLX1 antibody

This gene encodes a nuclear transcription factor that belongs to the NK-linked or NK-like (NKL) subfamily of homeobox genes. The encoded protein is required for normal development of the spleen during embryogenesis. This protein is also involved in specification of neuronal cell fates. Ectopic expression of this gene due to chromosomal translocations is associated with certain T-cell acute lymphoblastic leukemias. Alternate splicing results in multiple transcript variants.

Product Categories/Family for anti-TLX1 antibody

Antibodies; Transcription Factors; Zinc; Ring; Homeobox

References

De Keersmaecker, K., et al. Nat. Med. 16(11):1321-1327(2010). Milech, N., et al. Leuk. Res. 34(3):358-363(2010). Zweier-Renn, L.A., et al. Genes Chromosomes Cancer 49(2):119-131(2010). Riz, I., et al. Mol. Cancer 9, 181 (2010): Dear, T.N., et al. Proc. Natl. Acad. Sci. U.S.A. 90(10):4431-4435(1993).

NCBI and Uniprot Product Information

• NCBI GI #: 5031757
• NCBI GeneID: 3195
• NCBI Accession #: NP_005512.1
• NCBI GenBank Nucleotide #: NM_005521.3
• UniProt Accession #: P31314; O75699; Q5VXP2; Q9HCA0; Q9UD59; A1L4G3
• Molecular Weight: 26,245 Da
• NCBI Official Full Name: T-cell leukemia homeobox protein 1 isoform 1
• NCBI Official Synonym Full Names: T-cell leukemia homeobox 1
• NCBI Official Symbol: TLX1
• NCBI Official Synonym Symbols: TCL3; HOX11
• NCBI Protein Information: T-cell leukemia homeobox protein 1; proto-oncogene TCL-3; homeobox protein Hox-11; T-cell leukemia/lymphoma protein 3; homeo box-11 (T-cell leukemia-3 associated breakpoint, homologous to Drosophila Notch)
• UniProt Protein Name: T-cell leukemia homeobox protein 1
• Protein Family: T-cell leukemia homeobox protein
• UniProt Gene Name: TLX1
• UniProt Synonym Gene Names: HOX11; TCL3
• UniProt Entry Name: TLX1_HUMAN

NCBI Description

This gene encodes a nuclear transcription factor that belongs to the NK-linked or NK-like (NKL) subfamily of homeobox genes. The encoded protein is required for normal development of the spleen during embryogenesis. This protein is also involved in specification of neuronal cell fates. Ectopic expression of this gene due to chromosomal translocations is associated with certain T-cell acute lymphoblastic leukemias. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2010]

Uniprot Description

TLX1: Controls the genesis of the spleen. Binds to the DNA sequence 5'-GGCGGTAAGTGG-3'. A chromosomal aberration involving TLX1 may be a cause of a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(10;14)(q24;q11) with TCRD. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Oncoprotein

Chromosomal Location of Human Ortholog: 10q24

Cellular Component: nucleus

Molecular Function: protein binding; protein heterodimerization activity; sequence-specific DNA binding

Biological Process: neuron differentiation; spleen development; organ formation; cell fate commitment; central nervous system development; positive regulation of cell proliferation; positive regulation of transcription from RNA polymerase II promoter