Rabbit anti-Human SH3D20 Polyclonal Antibody | anti-SH3D20 antibody

SH3D20, ID (ARHGAP27, CAMGAP1, SH3D20, Rho GTPase-activating protein 27, CIN85-associated multi-domain-containing Rho GTPase-activating protein 1, Rho-type GTPase-activating protein 27, SH3 domain-containing protein 20) (Biotin)

Cat. Num. AAA6347254

• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: SH3D20; Polyclonal Antibody; ID (ARHGAP27; CAMGAP1; Rho GTPase-activating protein 27; CIN85-associated multi-domain-containing Rho GTPase-activating protein 1; Rho-type GTPase-activating protein 27; SH3 domain-containing protein 20) (Biotin); ARHGAP27; SH3 domain-containing protein 20; anti-SH3D20 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-SH3D20 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: SH3D20 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 197-226 amino acids from the Central region of human SH3D20.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(SH3D20 Antibody (Center) western blot analysis in A549,HepG2 cell line lysates (35ug/lane).This demonstrates the SH3D20 antibody detected the SH3D20 protein (arrow).)

Related Product Information for anti-SH3D20 antibody

SH3D20 is a rho GTPase-activating protein which may be involved in clathrin-mediated endocytosis. GTPase activators for the Rho-type GTPases act by converting them to an inactive GDP-bound state. Has activity toward CDC42 and RAC1 (By similarity).

Product Categories/Family for anti-SH3D20 antibody

Antibodies; GTPase; Rab; Ras; Rho Proteins

References

Edwards, T.L., et al. Ann. Hum. Genet. 74(2):97-109(2010).

NCBI and Uniprot Product Information

• NCBI GI #: 226693398
• NCBI GeneID: 201175; 201176
• UniProt Accession #: Q6ZUM4
• UniProt Protein Name: Rho GTPase-activating protein 27
• UniProt Gene Name: ARHGAP27
• UniProt Synonym Gene Names: CAMGAP1; SH3D20
• UniProt Entry Name: RHG27_HUMAN

Uniprot Description

Function: Rho GTPase-activating protein which may be involved in clathrin-mediated endocytosis. GTPase activators for the Rho-type GTPases act by converting them to an inactive GDP-bound state. Has activity toward CDC42 and RAC1

By similarity.

Subunit structure: Interacts with SH3KBP1/CIN85

By similarity.

Subcellular location: Cytoplasm

By similarity. Membrane; Peripheral membrane protein

By similarity.

Tissue specificity: Expressed in germinal center B-cell, spleen, chronic lymphocytic leukemia, pancreatic cancer and lung cancer. Ref.5

Sequence similarities: Contains 1 PH domain.Contains 1 Rho-GAP domain.Contains 1 SH3 domain.Contains 3 WW domains.

Caution: According to HGNC, ARHGAP27 and SH3D20 are 2 separate genes, corresponding to isoform 2 and isoform 4, respectively. However, a rat transcript and paralog proteins with a similar domain structure suggest the existence of a single gene encoding for a protein of 889 residues as displayed here.

Sequence caution: The sequence AAI01389.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.The sequence AAI01390.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.The sequence AAI01391.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.