Testing Data
(His-ubiquitin protein probed with H5099-16.)
Mouse His-Tag Monoclonal Antibody
His-Tag (6-Histidine, 6 His, 6-His Epitope Tag, HHHHHH Epitope Tag, HHHHHH Tag, Polyhistidine Tag) (APC)
Applications
Immunoprecipitation, Western Blot
Purity
Purified by protein G affinity chromatography from tissue culture supernatant.
Synonyms
His-Tag; Monoclonal Antibody; His-Tag (6-Histidine; 6 His; 6-His Epitope Tag; HHHHHH Epitope Tag; HHHHHH Tag; Polyhistidine Tag) (APC); Histidine Tag; anti-His-Tag antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
10D149
Specificity
Recognizes proteins containing the motif H-H-H-H-H-H and proteins containing histidine tags. Recognizes all His tagged proteins so far tested. Has been used to detect and purify histidine-tagged proteins expressed in mammalian and non-mammalian cell lines. In Western Blot of bacterial extracts, does not crossreact with any endogenous products, though some crossreactivity may be seen with extracts of insect or mammalian cells.
Purity/Purification
Purified by protein G affinity chromatography from tissue culture supernatant.
Form/Format
Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).
Applicable Applications for anti-His-Tag antibody
FLISA, Immunoprecipitation (IP), Western Blot (WB)
Immunogen
PAX6 transcription factor linked to 6-histidine tag (HHHHHH).
Conjugate
APC
Preparation and Storage
Store at 4 degree C. Do Not Freeze.
Testing Data
(His-ubiquitin protein probed with H5099-16.)
Testing Data
(His-ubiquitin protein probed with H5099-16.)
Product Categories/Family for anti-His-Tag antibody
References
1. Conrath, K., et al., J. Biol. Chem. 276: 7346-7350 (2001). 2. Suen, J.L., et al., Immunol. 103: 301-309 (2001). 3. Hoffmann, S.C., et al., J. Biol. Chem. 282: 22,370-22,375 (2007). 4. Zheng, J., et al., BMC Immunol. 8: 4 (2007). 5. Bahi, A. & Dreyer, J.L., Genes Brain Behav. 7: 244-256 (2008). 6. Wrighton, K.H., et al., J. Biol. Chem. 284: 9755-9763 (2009). 7. Diefenbacher, M., et al., J. Biol. Chem. 286: 25,027-25,038 (2011). 8. Alvarez, M.M., et al., PLoS One 5: e10176 (2010). 9. Bahi, A., et al., Neuropsychopharmacology 33: 2726-2734 (2008). 10. Gunnarsen, K.S., et al., BMC Biotechnol. 10: 8 (2010). 11. Hwang, H.Y., et al., Mol. Immunol. 45: 2570-2580 (2008). 12. De Vooght, L. et al. (2012) Microb Cell Fact. 11: 23. 13. Saerens, D. et al. (2004) J Biol Chem. 279 (50): 51965-72. 14. Than, N.G. et al. (2014) Placenta. 35: 855-65.
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Product Notes
The His-Tag (Catalog #AAA6268840) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's His-Tag can be used in a range of immunoassay formats including, but not limited to, FLISA, Immunoprecipitation (IP), Western Blot (WB). Researchers should empirically determine the suitability of the His-Tag for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "His-Tag, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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