Western Blot (WB)
(Western blot analysis of MYH13 in mouse extraocular muscle. The blot was probed on the left with anti-MYH13/Extraocular myosin (Hinge region) antibody (MP4571) and on the right with anti-MYH13/Extraocular myosin (C-terminal region) antibody (MP4561).)
Rabbit Myosin 13/Extraocular Myosin Polyclonal Antibody
Myosin 13/Extraocular Myosin (Hinge region) Antibody
Reactivity
Human, Rat, Mouse
Applications
Western Blot, ELISA, Immunohistochemistry
Synonyms
Myosin 13/Extraocular Myosin; Polyclonal Antibody; Myosin 13/Extraocular Myosin (Hinge region) Antibody; Myosin 13/Extraocular Myosin (Hinge region); Myosin HC13H; anti-Myosin 13/Extraocular Myosin antibody
Host
Rabbit
Reactivity
Human, Rat, Mouse
Clonality
Polyclonal
Specificity
This antibody was affinity purified using MYH13 (C-terminal region) peptide (without carrier). The antibody detects a 215kDa* protein corresponding to the apparent molecular mass of MYH13 on SDS-PAGE immunoblots of mouse extraocular muscle, and does not detect MYHs in mouse diaphragm, gastrocnemius, or brain.
Form/Format
Rabbit polyclonal, affinity-purified antibody is supplied in 100ul phosphate-buffered saline, 50% glycerol, 1mg/ml BSA, and 0.05% sodium azide.
Applicable Applications for anti-Myosin 13/Extraocular Myosin antibody
Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC)
Application Notes
ELISA: 1:2000
WB: 1:1000
IHC: 1:50
WB: 1:1000
IHC: 1:50
Immunogen
A synthetic peptide (coupled to KLH) corresponding to amino acid residues in the hinge region from mouse MYH13/extraocular myosin. This sequence has 100% identity with rat and human MYH13, and has low homology to other myosin family members.
Preparation and Storage
Store at -20 degree C. Stable for 1 year.
Western Blot (WB)
(Western blot analysis of MYH13 in mouse extraocular muscle. The blot was probed on the left with anti-MYH13/Extraocular myosin (Hinge region) antibody (MP4571) and on the right with anti-MYH13/Extraocular myosin (C-terminal region) antibody (MP4561).)
Western Blot (WB)
(Western blot analysis of MYH13 in mouse extraocular muscle. The blot was probed on the left with anti-MYH13/Extraocular myosin (Hinge region) antibody (MP4571) and on the right with anti-MYH13/Extraocular myosin (C-terminal region) antibody (MP4561).)
Related Product Information for anti-Myosin 13/Extraocular Myosin antibody
Myosin is a highly conserved, ubiquitous protein found in all eukaryotic cells, where it provides the motor function for diverse movements such as cytokinesis, phagocytosis, and muscle contraction. All myosins contain an amino-terminal motor/head domain and a carboxy-terminal tail domain. The class II myosins, consist of the conventional two-headed myosins that form filaments and are composed of two myosin heavy chain (MYH) subunits and four myosin light chain subunits. There are 15 MYH genes identified as Class II myosins and these include six skeletal muscle MYHs (MYH1, MYH2, MYH3, MYH4, MYH8, MYH13), three cardiac MYHs (MYH6, MYH7, MYH7B) and two non-muscle MYHs (MYH9, MYH10), as well as a smooth muscle MYH (MYH11).
References
Moncman, C.L. et al. (2011) Invest Ophthalmol Vis Sci. 52(7):3962.
Briggs, M.M. & Schachat, F. (2002). J Exp. Biol. 205:3133.
Berg, J.S. et al. (2001) Mol Biol Cell. 12:780.
Briggs, M.M. & Schachat, F. (2002). J Exp. Biol. 205:3133.
Berg, J.S. et al. (2001) Mol Biol Cell. 12:780.
NCBI and Uniprot Product Information
UniProt Accession #
Molecular Weight
215kDa
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Product Notes
The Myosin 13/Extraocular Myosin (Catalog #AAA474453) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Myosin 13/Extraocular Myosin (Hinge region) Antibody reacts with Human, Rat, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Myosin 13/Extraocular Myosin can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC). ELISA: 1:2000 WB: 1:1000 IHC: 1:50. Researchers should empirically determine the suitability of the Myosin 13/Extraocular Myosin for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Myosin 13/Extraocular Myosin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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