Immunofluorescence (IF)
(Fluorescent image of C2C12cells stained with Mouse Hmga2 Antibody (C-term). MBS9206750 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).)
Rabbit anti-Human, mouse Hmga2 Polyclonal Antibody | anti-Hmga2 antibody
Mouse Hmga2 Antibody (C-term)
WB~~1:1000
Immunofluorescence (IF)
(Fluorescent image of C2C12cells stained with Mouse Hmga2 Antibody (C-term). MBS9206750 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).)
Immunofluorescence (IF)
(Fluorescent image of C2C12cells stained with Mouse Hmga2 Antibody (C-term). MBS9206750 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).)
Western Blot (WB)
(Western blot analysis of lysates from HepG2, NCCIT, mouse NIH/3T3, mouse F9 cell line (from left to right), using Hmga2 Antibody (C-term). MBS9206750 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.)
Western Blot (WB)
(Western blot analysis of lysates from HepG2, NCCIT, mouse NIH/3T3, mouse F9 cell line (from left to right), using Hmga2 Antibody (C-term). MBS9206750 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.)
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NCBI and Uniprot Product Information
Uniprot Description
HMGA2: Functions as a transcriptional regulator. Functions in cell cycle regulation through CCNA2. Plays an important role in chromosome condensation during the meiotic G2/M transition of spermatocytes. Interacts with E4F1. Interacts with NEK2. Belongs to the HMGA family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: DNA-binding; Oncoprotein
Cellular Component: nuclear chromosome; chromatin; nucleus
Molecular Function: protein binding; DNA-(apurinic or apyrimidinic site) lyase activity; enzyme binding; DNA binding; AT DNA binding
Biological Process: transcription from RNA polymerase II promoter; somatic stem cell maintenance; cell proliferation in forebrain; positive regulation of multicellular organism growth; negative regulation of transcription from RNA polymerase II promoter; signal transduction; regulation of growth hormone secretion; regulation of transcription, DNA-dependent; meiotic cell cycle; base-excision repair; regulation of growth; positive regulation of cell proliferation; mitosis; negative regulation of steroid hormone receptor signaling pathway; transcription, DNA-dependent; adrenal gland development; male gonad development; cell cycle; DNA catabolic process, endonucleolytic; chromosome condensation; cell proliferation; pituitary gland development; cell division; negative regulation of astrocyte differentiation; positive regulation of transcription factor activity; spermatogenesis; positive regulation of transcription from RNA polymerase II promoter; negative regulation of JAK-STAT cascade
Research Articles on Hmga2
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Product Notes
The Hmga2 hmga2 (Catalog #AAA9206750) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Mouse Hmga2 Antibody (C-term) reacts with Human, mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Hmga2 can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), ELISA (EIA), Western Blot (WB). IF~~1:25 WB~~1:1000. Researchers should empirically determine the suitability of the Hmga2 hmga2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Hmga2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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