Helicase Like Transcription Factor Recombinant Protein | HLTF recombinant protein

Recombinant Helicase Like Transcription Factor (HLTF)

Cat. Num. AAA2030393

• Gene Names: HLTF; ZBU1; HLTF1; RNF80; HIP116; SNF2L3; HIP116A; SMARCA3
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >95%
• Synonyms: Helicase Like Transcription Factor; Recombinant Helicase Like Transcription Factor (HLTF); HLTF recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >95%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; ALMH ALTDLRKKNP NIKSLVVSQF TTFLSLIEIP LKASGFVFTR LDGSMAQKKR VESIQCFQNT EAGSPTIMLL SLKAGGVGLN LSAASRVFLM DPAWNPAAED QCFDRCHRLGQKQEVIITKF IVKDSVEENM LKIQNKKREL AAGAFGTKKP NADEMK
• Sequence Length: 1009

• Applicable Applications for HLTF recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 21.4kDa
• Accurate Molecular Mass (KD): 21kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: N-terminal His-Tag, T7 tag
• Organism Species: Homo sapiens (Human)
• Fragment: Ile255-Ser497
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 21071054
• NCBI GeneID: 6596
• NCBI Accession #: NP_620636.1
• NCBI GenBank Nucleotide #: NM_139048.2
• Molecular Weight: 99,919 Da
• NCBI Official Full Name: helicase-like transcription factor
• NCBI Official Synonym Full Names: helicase-like transcription factor
• NCBI Official Symbol: HLTF
• NCBI Official Synonym Symbols: ZBU1; HLTF1; RNF80; HIP116; SNF2L3; HIP116A; SMARCA3
• NCBI Protein Information: helicase-like transcription factor
• UniProt Protein Name: Helicase-like transcription factor
• Protein Family: Helicase-like transcription factor
• UniProt Gene Name: HLTF
• UniProt Synonym Gene Names: HIP116A; RNF80; SMARCA3; SNF2L3; ZBU1
• UniProt Entry Name: HLTF_HUMAN

NCBI Description

This gene encodes a member of the SWI/SNF family. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein contains a RING finger DNA binding motif. Two transcript variants encoding the same protein have been found for this gene. However, use of an alternative translation start site produces an isoform that is truncated at the N-terminus compared to the full-length protein. [provided by RefSeq, Jul 2008]

Uniprot Description

SMARCA3: an enzyme with apparent ATP-dependent DNA helicase activity. Belongs to the SNF2/RAD54 helicase family, RAD16 subfamily. Two alternatively spliced human isoforms have been described.

Protein type: EC 6.3.2.-; Helicase; EC 3.6.4.-; EC 3.6.1.-; Ligase; EC 6.3.2.19; DNA-binding; Nucleolus; Ubiquitin ligase; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 3q25.1-q26.1

Cellular Component: nucleoplasm; membrane; cytoplasm; nucleolus; nucleus

Molecular Function: protein binding; DNA binding; zinc ion binding; ATPase activity; helicase activity; ATP binding; ligase activity

Biological Process: regulation of transcription from RNA polymerase II promoter; protein polyubiquitination; regulation of transcription, DNA-dependent; transcription, DNA-dependent; postreplication repair; chromatin modification