HLA-C recombinant protein

Recombinant Human HLA-C protein

Cat. Num. AAA1561611

• Gene Names: HLA-C; HLC-C; D6S204; PSORS1; HLA-JY3
• Purity: >90% as determined by SDS-PAGE
• Synonyms: HLA-C; Recombinant Human HLA-C protein; HLA class I histocompatibility antigen; C alpha chain; HLA-Cw; Human leukocyte antigen C; HLA-C recombinant protein

• Host: E Coli
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized. Lyophilized from PBS pH 7.4, 0.2% NLS, 5% Trehalose, 5% Mannose, 1mM EDTA.

• Species: Homo sapiens (Human)
• Protein Construction: A DNA sequence encoding the human HLA-C (Ser48-Thr305) was fused with His tag
• Reconstitution: Reconstitute in sterile water for a stock solution.
• Preparation and Storage: Store at 2 to 8 degree C for one week. Store at -20 to -80 degree C for twelve months from the date of receipt. Use a manual defrost freezer and avoid repeated freeze thaw cycles.; In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.

Related Product Information for HLA-C recombinant protein

Antigen-presenting major histocompatibility complex class I (MHCI) molecule with an important role in reproduction and antiviral immunity. In complex with B2M/beta 2 microglobulin displays a restricted repertoire of self and viral peptides and acts as a dominant ligand for inhibitory and activating killer immunoglobulin receptors (KIRs) expressed on NK cells. In an allogeneic setting, such as during pregnancy, mediates interaction of extravillous trophoblasts with KIR on uterine NK cells and regulate trophoblast invasion necessary for placentation and overall fetal growth. During viral infection, may present viral peptides with low affinity for KIRs, impeding KIR-mediated inhibition through peptide antagonism and favoring lysis of infected cells. Presents a restricted repertoire of viral peptides on antigen-presenting cells for recognition by alpha-beta T cell receptor (TCR) on HLA-C-restricted CD8-positive T cells, guiding antigen-specific T cell immune response to eliminate infected cells, particularly in chronic viral infection settings such as HIV-1 or CMV infection. Both the peptide and the MHC molecule are recognized by TCR, the peptide is responsible for the fine specificity of antigen recognition and MHC residues account for the MHC restriction of T cells. Typically presents intracellular peptide antigens of 9 amino acids that arise from cytosolic proteolysis via proteasome. Can bind different peptides containing allele-specific binding motifs, which are mainly defined by anchor residues at position 2 and 9. Preferentially displays peptides having a restricted repertoire of hydrophobic or aromatic amino acids (Phe, Ile, Leu, Met, Val and Tyr) at the C-terminal anchor.

Product Categories/Family for HLA-C recombinant protein

Protein

NCBI and Uniprot Product Information

• NCBI GI #: 52630342
• NCBI GeneID: 3107
• NCBI Accession #: NP_002108
• NCBI GenBank Nucleotide #: NM_002117.5
• UniProt Accession #: P10321; O62883; O78060; O78068; O78069; P30503; Q29743; Q29992; Q29993; Q9GIK8; Q9TQB4; P04222; P30499; P30501; P30504; P30505; P30508; P30510; Q29960; Q29963
• Molecular Weight: 40,861 Da
• NCBI Official Full Name: HLA class I histocompatibility antigen, Cw-1 alpha chain
• NCBI Official Synonym Full Names: major histocompatibility complex, class I, C
• NCBI Official Symbol: HLA-C
• NCBI Official Synonym Symbols: HLC-C; D6S204; PSORS1; HLA-JY3
• NCBI Protein Information: HLA class I histocompatibility antigen, Cw-1 alpha chain; HLA class I histocompatibility antigen, C alpha chain; MHC class I antigen heavy chain HLA-C; human leukocyte antigen-C alpha chain; major histocompatibility antigen HLA-C
• UniProt Protein Name: HLA class I histocompatibility antigen, Cw-7 alpha chain
• Protein Family: HLA class I histocompatibility antigen
• UniProt Gene Name: HLA-C
• UniProt Synonym Gene Names: HLAC

NCBI Description

HLA-C belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domain, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Over one hundred HLA-C alleles have been described [provided by RefSeq, Jul 2008]

Uniprot Description

Involved in the presentation of foreign antigens to the immune system.