Mouse HLA-C Antibody | anti-HLA-C antibody

Anti-HLA-C Purified

Cat. Num. AAA1802252

• Gene Names: HLA-C; HLC-C; D6S204; PSORS1; HLA-JY3
• Reactivity: Non-human primates, Human
• Applications: Immunoprecipitation, Flow Cytometry, Functional Assay
• Purity: Purified by protein-A affinity chromatography.
• Synonyms: HLA-C; Anti-HLA-C Purified; HLC-C; D6S204; PSORS1; HLA-JY3; major histocompatibility complex; class I; C; anti-HLA-C antibody

• Host: Mouse
• Reactivity: Non-human primates, Human
• Isotype: Mouse IgG2b
• Clone Number: DT-9
• Specificity: The mouse monoclonal antibody DT-9 recognizes an extracellular epitope on HLA-C member of MHC class I molecules. It does not crossreact with HLA-A or HLA-B allotypes.
• Purity/Purification: Purified by protein-A affinity chromatography.
• Form/Format: Phosphate buffered saline (PBS), pH7.4, 15mM sodium azide
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-HLA-C antibody: Immunoprecipitation (IP), Flow Cytometry (FC/FACS)(QC tested)
• Application Notes: FC/FACS: Recommended dilution: 1-4ug/ml.
• Immunogen: Purified MHC class I molecules of tamarin origin
• Preparation and Storage: Store at 2-8 degree C. Do not freeze.

Flow Cytometry (FC/FACS)

(Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human HLA-C (DT-9) purified antibody (concentration in sample 1,7ug/ml, GAM APC).)

Flow Cytometry (FC/FACS)

(Separation of lymphocytes stained anti-human HLA-C (DT-9) purified antibody (concentration in sample 1,7ug/ml, GAM APC, red-filled) from lymphocytes unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (surface staining).)

Related Product Information for anti-HLA-C antibody

HLA-C, a member of MHC class I glycoproteins, is one of polymorphysm typing targets, which are important for transplantation. The HLA system plays an important role in the occurrence and outcome of infectious diseases. The structural spike and the nucleocapsid proteins of the novel coronavirus SARS-CoV-2, which causes coronavirus disease 2019 (COVID-19), are reported to contain multiple Class I epitopes with predicted HLA restrictions. Individual HLA genetic variation may help explain different immune responses to a virus across a population. It has been described that HLA-C interacts with human herpesvirus 8 MIR1 protein.

References

Korner C, Simoneau CR, Schommers P, Granoff M, Ziegler M, Hölzemer A, Lunemann S, Chukwukelu J, Corleis B, Naranbhai V, Kwon DS, Scully EP, Jost S, Kirchhoff F, Carrington M, Altfeld M: HIV-1-Mediated Downmodulation of HLA-C Impacts Target Cell Recognition and Antiviral Activity of NK Cells. Cell Host Microbe 2017 Jul 12;22(1):111-119., URL: https://pubmed.ncbi.nlm.nih.gov/28704647/
Tremante E, Lo Monaco E, Ingegnere T, Sampaoli C, Fraioli R, Giacomini P: Monoclonal antibodies to HLA-E bind epitopes carried by unfolded beta2 m-free heavy chains. Eur J Immunol. 2015 Aug;45(8):2356-64., URL: https://pubmed.ncbi.nlm.nih.gov/25982269/
Serena M, Parolini F, Biswas P, Sironi F, Blanco Miranda A, Zoratti E, Scupoli MT, Ziglio S, Valenzuela-Fernandez A, Gibellini D, Romanellimg, Siccardi A, Malnati M, Beretta A, Zipeto D: HIV-1 Env associates with HLA-C free-chains at the cell membrane modulating viral infectivity. Sci Rep. 2018 May 22;8:46991. doi: 10.1038/srep46991., URL: https://pubmed.ncbi.nlm.nih.gov/29786690/
Corrah TW, Goonetilleke N, Kopycinski J, Deeks SG, Cohen MS, Borrow P, McMichael A, Brackenridge S: Reappraisal of the relationship between the HIV-1-protective single-nucleotide polymorphism 35 kilobases upstream of the HLA-C gene and surface HLA-C expression. J Virol. 2011 Apr;85(7):3367-74., URL: https://pubmed.ncbi.nlm.nih.gov/21248048/
van der Ploeg K, Chang C, Ivarsson MA, Moffett A, Wills MR, Trowsdale J: Modulation of human leukocyte antigen-C by human cytomegalovirus stimulates KIR2DS1 recognition by natural killer cells. Front Immunol. 2017 Mar 29;8:298., URL: https://pubmed.ncbi.nlm.nih.gov/28424684/
Parolini F, Biswas P, Serena M, Sironi F, Muraro V, Guizzardi E, Cazzoletti L, Scupoli MT, Gibellini D, Ugolotti E, Biassoni R, Beretta A, Malnati M, Romanellimg, Zipeto D: Stability and expression levels of HLA-C on the cell membrane modulate HIV-1 infectivity. J Virol. 2017 Dec 14;92(1):e01711-17., URL: https://pubmed.ncbi.nlm.nih.gov/29070683/

NCBI and Uniprot Product Information

• NCBI GI #: 52630342
• NCBI GeneID: 3107
• NCBI Accession #: NP_002108
• NCBI GenBank Nucleotide #: NM_002117.5
• UniProt Accession #: P10321; O62883; O78060; O78068; O78069; P30503; Q29743; Q29992; Q29993; Q9GIK8; Q9TQB4; P04222; P30499; P30501; P30504; P30505; P30508; P30510; Q29960; Q29963
• Molecular Weight: 40,861 Da
• NCBI Official Full Name: HLA class I histocompatibility antigen, Cw-1 alpha chain
• NCBI Official Synonym Full Names: major histocompatibility complex, class I, C
• NCBI Official Symbol: HLA-C
• NCBI Official Synonym Symbols: HLC-C; D6S204; PSORS1; HLA-JY3
• NCBI Protein Information: HLA class I histocompatibility antigen, Cw-1 alpha chain; HLA class I histocompatibility antigen, C alpha chain; MHC class I antigen heavy chain HLA-C; human leukocyte antigen-C alpha chain; major histocompatibility antigen HLA-C
• UniProt Protein Name: HLA class I histocompatibility antigen, Cw-7 alpha chain
• Protein Family: HLA class I histocompatibility antigen
• UniProt Gene Name: HLA-C
• UniProt Synonym Gene Names: HLAC

NCBI Description

HLA-C belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domain, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Over one hundred HLA-C alleles have been described [provided by RefSeq, Jul 2008]

Uniprot Description

Involved in the presentation of foreign antigens to the immune system.