Goat Hepatocyte growth factor receptor ELISA Kit | HGFR elisa kit

Goat Hepatocyte growth factor receptor ELISA Kit

Cat. Num. AAA735328

• Gene Names: MET; HGFR; AUTS9; RCCP2; c-Met
• Reactivity: Goat
• Synonyms: Hepatocyte growth factor receptor; Goat Hepatocyte growth factor receptor ELISA Kit; HGFR elisa kit

• Reactivity: Goat

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 1.0 pg/mL.
• Intended Uses: This HGFR ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Goat HGFR. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for HGFR elisa kit

Principle of the assay: HGFR ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-HGFR antibody and an HGFR-HRP conjugate. The assay sample and buffer are incubated together with HGFR-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the HGFR concentration since HGFR from samples and HGFR-HRP conjugate compete for the anti-HGFR antibody binding site. Since the number of sites is limited, as more sites are occupied by HGFR from the sample, fewer sites are left to bind HGFR-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HGFR concentration in each sample is interpolated from this standard curve.

Product Categories/Family for HGFR elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 188595716
• NCBI GeneID: 4233
• NCBI Accession #: NP_001120972.1
• NCBI GenBank Nucleotide #: NM_001127500.1
• Molecular Weight: 85,745 Da
• NCBI Official Full Name: hepatocyte growth factor receptor isoform a
• NCBI Official Synonym Full Names: MET proto-oncogene, receptor tyrosine kinase
• NCBI Official Symbol: MET
• NCBI Official Synonym Symbols: HGFR; AUTS9; RCCP2; c-Met
• NCBI Protein Information: hepatocyte growth factor receptor; SF receptor; HGF receptor; HGF/SF receptor; proto-oncogene c-Met; scatter factor receptor; tyrosine-protein kinase Met; met proto-oncogene tyrosine kinase
• UniProt Protein Name: Hepatocyte growth factor receptor
• Protein Family: Hepatocyte growth factor receptor
• UniProt Gene Name: MET
• UniProt Synonym Gene Names: HGF receptor; SF receptor
• UniProt Entry Name: MET_HUMAN

NCBI Description

The proto-oncogene MET product is the hepatocyte growth factor receptor and encodes tyrosine-kinase activity. The primary single chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are disulfide linked to form the mature receptor. Various mutations in the MET gene are associated with papillary renal carcinoma. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Met: a proto-oncogenic receptor tyrosine kinase with high affinity for hepatocyte growth factor. The primary single chain precursor protein is post-translationally cleaved to produce the 45 kDa alpha- and 145 kDa beta-subunits, which are disulfide linked to form the mature receptor. Ligand-binding induces autophosphorylation at multiple tyrosines, which recruit several downstream signaling components, including Gab1, c-Cbl and PI3 kinase. Activating point mutations cause hereditary papillary renal carcinoma. Mutations also seen in sporadic renal cell carcinoma and childhood hepatocellular carcinoma. Upregulation in carcinomas and sarcomas correlates with metastasis and poor outcome. Some gastric carcinomas harbor a translocation that creates an activated TPR-Met fusion protein. A small molecule inhibitor (PHA-665752) shows an effect in gastric carcinoma xenografts. Inhibitors: SU11274, PHA-665752, mAbs. Two alternatively spliced human isoforms have been reported.

Protein type: Oncoprotein; EC 2.7.10.1; Protein kinase, tyrosine (receptor); Membrane protein, integral; Protein kinase, TK; Kinase, protein; TK group; Met family

Chromosomal Location of Human Ortholog: 7q31

Cellular Component: cell surface; integral to plasma membrane; extracellular region; plasma membrane; integral to membrane; basal plasma membrane

Molecular Function: protein binding; protein-tyrosine kinase activity; protein phosphatase binding; hepatocyte growth factor receptor activity; ATP binding

Biological Process: axon guidance; skeletal muscle development; peptidyl-tyrosine phosphorylation; activation of MAPK activity; protein amino acid autophosphorylation; myoblast proliferation; myotube differentiation; muscle cell migration; hepatocyte growth factor receptor signaling pathway; liver development; glucose homeostasis; signal transduction; cell proliferation; cell surface receptor linked signal transduction; branching morphogenesis of a tube; positive chemotaxis; adult behavior; positive regulation of transcription from RNA polymerase II promoter; brain development; endothelial cell morphogenesis; placenta development

Disease: Hepatocellular Carcinoma; Renal Cell Carcinoma, Papillary, 1