Human Hepatocyte growth factor receptor ELISA Kit | HGFR elisa kit

Human Hepatocyte growth factor receptor ELISA Kit

Cat. Num. AAA725798

• Gene Names: MET; HGFR; AUTS9; RCCP2; c-Met
• Reactivity: Human
• Synonyms: Hepatocyte growth factor receptor; Human Hepatocyte growth factor receptor ELISA Kit; HGFR elisa kit

• Reactivity: Human

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive or Sandwich
• Detection Range: 250 -5000pg/mL
• Sensitivity: 1.0pg/mL
• Intended Uses: This HGFR ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human HGFR. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for HGFR elisa kit

Principle of the Assay: HGFR ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for HGFR. Standards or samples are then added to the microtiter plate wells and HGFR if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of HGFR present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for HGFR are added to each well to "sandwich" the HGFR immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain HGFR and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HGFR concentration in each sample is interpolated from this standard curve.

Product Categories/Family for HGFR elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 188595716
• NCBI GeneID: 4233
• NCBI Accession #: NP_001120972.1
• NCBI GenBank Nucleotide #: NM_001127500.1
• Molecular Weight: 85,745 Da
• NCBI Official Full Name: hepatocyte growth factor receptor isoform a
• NCBI Official Synonym Full Names: MET proto-oncogene, receptor tyrosine kinase
• NCBI Official Symbol: MET
• NCBI Official Synonym Symbols: HGFR; AUTS9; RCCP2; c-Met
• NCBI Protein Information: hepatocyte growth factor receptor; SF receptor; HGF receptor; HGF/SF receptor; proto-oncogene c-Met; scatter factor receptor; tyrosine-protein kinase Met; met proto-oncogene tyrosine kinase
• UniProt Protein Name: Hepatocyte growth factor receptor
• Protein Family: Hepatocyte growth factor receptor
• UniProt Gene Name: MET
• UniProt Synonym Gene Names: HGF receptor; SF receptor
• UniProt Entry Name: MET_HUMAN

NCBI Description

The proto-oncogene MET product is the hepatocyte growth factor receptor and encodes tyrosine-kinase activity. The primary single chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are disulfide linked to form the mature receptor. Various mutations in the MET gene are associated with papillary renal carcinoma. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Met: a proto-oncogenic receptor tyrosine kinase with high affinity for hepatocyte growth factor. The primary single chain precursor protein is post-translationally cleaved to produce the 45 kDa alpha- and 145 kDa beta-subunits, which are disulfide linked to form the mature receptor. Ligand-binding induces autophosphorylation at multiple tyrosines, which recruit several downstream signaling components, including Gab1, c-Cbl and PI3 kinase. Activating point mutations cause hereditary papillary renal carcinoma. Mutations also seen in sporadic renal cell carcinoma and childhood hepatocellular carcinoma. Upregulation in carcinomas and sarcomas correlates with metastasis and poor outcome. Some gastric carcinomas harbor a translocation that creates an activated TPR-Met fusion protein. A small molecule inhibitor (PHA-665752) shows an effect in gastric carcinoma xenografts. Inhibitors: SU11274, PHA-665752, mAbs. Two alternatively spliced human isoforms have been reported.

Protein type: EC 2.7.10.1; Protein kinase, tyrosine (receptor); Membrane protein, integral; Protein kinase, TK; Oncoprotein; Kinase, protein; TK group; Met family

Chromosomal Location of Human Ortholog: 7q31

Cellular Component: cell surface; integral to plasma membrane; plasma membrane; extracellular region; integral to membrane; basal plasma membrane

Molecular Function: protein binding; protein-tyrosine kinase activity; hepatocyte growth factor receptor activity; protein phosphatase binding; ATP binding

Biological Process: axon guidance; skeletal muscle development; peptidyl-tyrosine phosphorylation; activation of MAPK activity; myoblast proliferation; protein amino acid autophosphorylation; myotube differentiation; muscle cell migration; hepatocyte growth factor receptor signaling pathway; glucose homeostasis; liver development; signal transduction; cell proliferation; cell surface receptor linked signal transduction; positive chemotaxis; branching morphogenesis of a tube; adult behavior; positive regulation of transcription from RNA polymerase II promoter; endothelial cell morphogenesis; brain development; placenta development

Disease: Hepatocellular Carcinoma; Renal Cell Carcinoma, Papillary, 1