Rabbit anti-Human ELF3 Polyclonal Antibody | anti-ELF3 antibody

ELF3, CT (ELF3, ERT, ESX, JEN, ETS-related transcription factor Elf-3, E74-like factor 3, Epithelial-restricted with serine box, Epithelium-restricted Ets protein ESX, Epithelium-specific Ets transcription factor 1) (PE)

Cat. Num. AAA6295214

• Gene Names: ELF3; ERT; ESX; EPR-1; ESE-1
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: ELF3; Polyclonal Antibody; CT (ELF3; ERT; ESX; JEN; ETS-related transcription factor Elf-3; E74-like factor 3; Epithelial-restricted with serine box; Epithelium-restricted Ets protein ESX; Epithelium-specific Ets transcription factor 1) (PE); Epithelium-specific Ets transcription factor 1; anti-ELF3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-ELF3 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: ELF3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 328~358 amino acids from the C-terminal region of human ELF3.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of ELF3 Antibody (C-term) in MCF-7 cell line lysates (35ug/lane). ELF3 (arrow) was detected using the purified Pab.)

Product Categories/Family for anti-ELF3 antibody

Antibodies; Transcription Factors

References

Lee, S.H., et al. Mol. Cancer Ther. 7(12):3739-3750(2008). Wu, J., et al. Cell Res. 18(6):649-663(2008). Peng, H., et al. J. Cell. Physiol. 215(2):562-573(2008). Iwai, S., et al. Oral Dis 14(2):144-149(2008). Manavathi, B., et al. J. Biol. Chem. 282(27):19820-19830(2007). Choi, S.G., et al. J. Biol. Chem. 273(1):110-117(1998).

NCBI and Uniprot Product Information

• NCBI GI #: 167235022
• NCBI GeneID: 1999
• NCBI Accession #: NP_004424.3
• NCBI GenBank Nucleotide #: NM_004433
• UniProt Accession #: P78545; Q6IAP8; Q7RU03; Q7Z3X2; Q8NFG2; Q99718; B2R5Q6
• Molecular Weight: 39,357 Da
• NCBI Official Full Name: Homo sapiens E74-like factor 3 (ets domain transcription factor, epithelial-specific) (ELF3), transcript variant 1, mRNA
• NCBI Official Synonym Full Names: E74-like factor 3 (ets domain transcription factor, epithelial-specific )
• NCBI Official Symbol: ELF3
• NCBI Official Synonym Symbols: ERT; ESX; EPR-1; ESE-1
• NCBI Protein Information: ETS-related transcription factor Elf-3; E74-like factor 3 (ETS domain transcription factor, serine box, epithelial-specific); E74-like factor 3 (ets domain transcription factor); ELF3; epithelial-restricted with serine box; epithelium-restricted Ets prote
• UniProt Protein Name: ETS-related transcription factor Elf-3
• Protein Family: ELF3-like protein
• UniProt Gene Name: ELF3
• UniProt Synonym Gene Names: ESE-1
• UniProt Entry Name: ELF3_HUMAN

Uniprot Description

ELF3: Transcriptional activator that binds and transactivates ETS sequences containing the consensus nucleotide core sequence GGA[AT]. Acts synergistically with POU2F3 to transactivate the SPRR2A promoter and with RUNX1 to transactivate the ANGPT1 promoter. Also transactivates collagenase, CCL20, CLND7, FLG, KRT8, NOS2, PTGS2, SPRR2B, TGFBR2 and TGM3 promoters. Represses KRT4 promoter activity. Involved in mediating vascular inflammation. May play an important role in epithelial cell differentiation and tumorigenesis. May be a critical downstream effector of the ERBB2 signaling pathway. May be associated with mammary gland development and involution. Plays an important role in the regulation of transcription with TATA-less promoters in preimplantation embryos, which is essential in preimplantation development. Belongs to the ETS family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor; Motility/polarity/chemotaxis; DNA-binding

Chromosomal Location of Human Ortholog: 1q32.2

Cellular Component: cytoplasm; nucleus

Molecular Function: protein binding; transcription coactivator activity; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; regulation of transcription from RNA polymerase II promoter; mammary gland involution; epidermis development; extracellular matrix organization and biogenesis; blastocyst development; epithelial cell differentiation; positive regulation of transcription, DNA-dependent; multicellular organismal development; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; inflammatory response