HGFA recombinant protein

Recombinant Human HGFA Protein (His tag)

Cat. Num. AAA2546333

• Gene Names: HGFAC; HGFA
• Purity: > 95 % as determined by SDS-PAGE
• Synonyms: HGFA; Recombinant Human HGFA Protein (His tag); RP11-529E10.2; MGC138395; MGC138397; HGFA recombinant protein

• Host: Human Cells
• Purity/Purification: > 95 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 655

• Application Notes: The recombinant human HGF activator consists of 631 amino acids and has a predicted molecular mass of 68.2 kDa. As a result of proteolysis or autocatalysis and glycosylation, it migrates as four bands (34, 37, 65, 105 kDa) in SDS-PAGE under reducing conditions, corresponding to the active long chain, uncleaved single chain (long chain + short chain), pro doamin and the full-length pro form of HGFA respectively.
• Predicted N Terminal: Gln 36
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

Related Product Information for HGFA recombinant protein

Background: HGF activator (HGFA) is a serum-derived serine protease and belongs to the peptidase family S1.HGFA is responsible for the conversion of hepatocyte growth factor (HGF), from the inactive single-chain precursor to the active heterodimeric form, which is a potent mitogen, motogen, and morphogen for liver cells, epithelial cells, and endothelial cells. HGFA is synthesized and secreted by the liver and circulates in the plasma as an inactive single-chain zymogen in normal states. The zymogen is cleaved by thrombin or thermolysin through the endoproteolytic process and forms an active heterodimer linked by a disulfide bond. In turn, the active protease can be inhibited by HGFA inhibitors (HAIs) including HAI-1 and HAI-2. In addition, the HGFA zymogen acquires a strong affinity upon activation and thus may ensure the local action in tissue regeneration in liver, kidney and skin. It has been reported that activation of HGF is a critical limiting step in the HGF/SF-induced signaling pathway mediated by Met, and accordingly, aberrant expression of HGFA is implicated in tumorigenesis and progression.

Description: A DNA sequence encoding the human HGF activator precursor (NP_001519.1) (Met 1-Ser 655) was expressed with a fused polyhistidine-tag at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 4504383
• NCBI GeneID: 3083
• NCBI Accession #: NP_001519.1
• NCBI GenBank Nucleotide #: NM_001528.3
• UniProt Accession #: Q04756; Q14726; Q2M1W7; Q53X47
• Molecular Weight: 70,682 Da
• NCBI Official Full Name: hepatocyte growth factor activator isoform 2 preproprotein
• NCBI Official Synonym Full Names: HGF activator
• NCBI Official Symbol: HGFAC
• NCBI Official Synonym Symbols: HGFA
• NCBI Protein Information: hepatocyte growth factor activator
• UniProt Protein Name: Hepatocyte growth factor activator
• UniProt Gene Name: HGFAC
• UniProt Synonym Gene Names: HGF activator; HGFA

NCBI Description

This gene encodes a member of the peptidase S1 protein family. The encoded protein is first synthesized as an inactive single-chain precursor before being activated to a heterodimeric form by endoproteolytic processing. It acts as serine protease that converts hepatocyte growth factor to the active form. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]

Uniprot Description

HGFAC: Activates hepatocyte growth factor (HGF) by converting it from a single chain to a heterodimeric form. Belongs to the peptidase S1 family.

Protein type: EC 3.4.21.-; Protease; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 4p16.3

Molecular Function: serine-type peptidase activity