Mouse Glycogen phosphorylase BB (GP-BB) ELISA Kit | GP-BB elisa kit

Mouse Glycogen phosphorylase BB (GP-BB) ELISA Kit

Cat. Num. AAA268065

• Gene Names: PYGB; GPBB
• Reactivity: Mouse
• Synonyms: Glycogen phosphorylase BB (GP-BB); Mouse Glycogen phosphorylase BB (GP-BB) ELISA Kit; GP-BB elisa kit

• Reactivity: Mouse
• Sequence Length: 843

• Samples: Mouse serum, plasma or cell culture supernatant
• Assay Type: Quantitative Sandwich
• Detection Range: 20 ng/mL-0.312 ng/mL
• Sensitivity: Up to 0.06 ng/mL
• Intra-assay Precision: <= 8%
• Inter-assay Precision: <= 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

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Related Product Information for GP-BB elisa kit

Principle of the Assay: As mentioned above, this kit utilizes the Double Antibody Sandwich ELISA technique. The pre-coated antibody is an anti-Mouse GP-BB monoclonal antibody, while the detection antibody is a biotinylated polyclonal antibody. Samples and biotinylated antibodies are added into ELISA plate wells and washed out with PBS or TBS after their respective additions to the wells. Then Avidin-peroxidase conjugates are added to the wells in after. TMB substrate is used for coloration after the enzyme conjugate has already been thoroughly washed out of the wells by PBS or TBS. TMB reacts to form a blue product from the peroxidase activity, and finally turns to yellow after addition of the stop solution (Color Reagent C). The color intensity and quantity of target analyte in the sample are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 21361370
• NCBI GeneID: 5834
• NCBI Accession #: NP_002853.2
• NCBI GenBank Nucleotide #: NM_002862.3
• Molecular Weight: 96,696 Da
• NCBI Official Full Name: glycogen phosphorylase, brain form
• NCBI Official Synonym Full Names: phosphorylase, glycogen; brain
• NCBI Official Symbol: PYGB
• NCBI Official Synonym Symbols: GPBB
• NCBI Protein Information: glycogen phosphorylase, brain form; glycogen phosphorylase B
• UniProt Protein Name: Glycogen phosphorylase, brain form
• UniProt Gene Name: PYGB
• UniProt Entry Name: PYGB_HUMAN

NCBI Description

The protein encoded by this gene is a glycogen phosphorylase found predominantly in the brain. The encoded protein forms homodimers which can associate into homotetramers, the enzymatically active form of glycogen phosphorylase. The activity of this enzyme is positively regulated by AMP and negatively regulated by ATP, ADP, and glucose-6-phosphate. This enzyme catalyzes the rate-determining step in glycogen degradation. [provided by RefSeq, Jul 2008]

Uniprot Description

PYGB: Phosphorylase is an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties. Belongs to the glycogen phosphorylase family.

Protein type: EC 2.4.1.1; Kinase, other; Carbohydrate Metabolism - starch and sucrose; Transferase; Phosphorylase

Chromosomal Location of Human Ortholog: 20p11.21

Cellular Component: membrane; cytoplasm

Molecular Function: glycogen phosphorylase activity; pyridoxal phosphate binding

Biological Process: glycogen catabolic process; carbohydrate metabolic process; glucose metabolic process; pathogenesis