Rabbit anti-Mouse HGF Polyclonal Antibody | anti-HGF antibody

Anti-HGF Antibody, Rabbit Polyclonal

Cat. Num. AAA8110836

• Gene Names: Hgf; SF; NK1; NK2; HGF/SF; SF/HGF; C230052L06Rik
• Reactivity: Mouse
• Applications: ELISA, Immunohistochemistry
• Purity: Protein A & Antigen Affinity
• Synonyms: HGF; Polyclonal Antibody; Anti-HGF Antibody; Rabbit Polyclonal; HGF/Hepatocyte Growth Factor Antibody; Rabbit PAb; Antigen Affinity Purified; hepatocyte growth factor (hepapoietin A; scatter factor); Anti-C230052L06Rik Antibody; Anti-HGF/SF Antibody; Anti-NK1 Antibody; Anti-NK2 Antibody; Anti-SF Antibody; Anti-SF/HGF Antibody; anti-HGF antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Mouse HGF/Hepatocyte Growth Factor
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-HGF antibody: ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: ELISA: 1:5000-1:10,000; IHC-P: 1:1000-1:4000
• Immunogen: Recombinant Mouse HGF/Hepatocyte Growth Factor protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Mouse HGF/Hepatocyte Growth Factor. HGF/Hepatocyte Growth Factor specific IgG was purified by Mouse HGF/Hepatocyte Growth Factor affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunochemical staining of mouse HGF in mouse liver with rabbit polyclonal antibody (1:2000, formalin-fixed paraffin embedded sections).)

Immunohistochemistry (IHC)

(Immunochemical staining of mouse HGF in mouse spleen with rabbit polyclonal antibody (1:2000, formalin-fixed paraffin embedded sections).)

Related Product Information for anti-HGF antibody

Hepatocyte growth factor, also known as HGF, contains 4 kringle domains, 1 PAN domain, and 1 peptidase S1 domain. It belongs to the peptidase S1 family, plasminogen subfamily. The hepatocyte growth factor is secreted by mesenchymal cells as a single inactive polypeptide and is cleaved by serine proteases into a 69-kDa alpha-chain and 34-kDa beta-chain. A disulfide bond between the alpha and beta chains produces the active, heterodimeric molecule. The hepatocyte growth factor regulates cell growth, cell motility, and morphogenesis by activating a tyrosine kinase signaling cascade after binding to the proto-oncogenic c-Met receptor, and acts as a multi-functional cytokine on cells of mainly epithelial origin. Its ability to stimulate mitogenesis, cell motility and matrix invasion give it a central role in angiogenesis, tumorogenesis, and tissue regeneration. HGF is a potent mitogen for mature parenchymal hepatocyte cells, seems to be an hepatotrophic factor, and acts as a growth factor for a broad spectrum of tissues and cell types. HGF has no detectable protease activity. Defects in hepatocyte growth factor are the cause of deafness autosomal recessive type 39. A form of profound prelingual sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information.

Product Categories/Family for anti-HGF antibody

Cancer Drug Targets

References

Naldini L, et al. (1991) Scatter factor and hepatocyte growth factor are indistinguishable ligands for the MET receptor. EMBO J. 10(10):2867-78.
Comoglio, et al. (1993) Structure, biosynthesis and biochemical properties of the HGF receptor in normal and malignant cells. 65:131-65.
Hahn W, et al. (2011) Enhanced cardioprotective effects by coexpression of two isoforms of hepatocyte growth factor from naked plasmid DNA in a rat ischemic heart disease model. The Journal of Gene Medicine. 13(10):549-55.
Bottaro DP, et al. (1991) Identification of the hepatocyte growth factor receptor as the c-met proto-oncogene product. Science. 251(4995):802-4.

NCBI and Uniprot Product Information

• NCBI GI #: 575501559
• NCBI GeneID: 15234
• NCBI Accession #: NP_001276387.1
• NCBI GenBank Nucleotide #: NM_001289458.1
• UniProt Accession #: Q08048; O55027; Q53WS5; Q61662; Q64007; Q6LBE6
• Molecular Weight: 24,308 Da
• NCBI Official Full Name: hepatocyte growth factor isoform 1 preproprotein
• NCBI Official Synonym Full Names: hepatocyte growth factor
• NCBI Official Symbol: Hgf
• NCBI Official Synonym Symbols: SF; NK1; NK2; HGF/SF; SF/HGF; C230052L06Rik
• NCBI Protein Information: hepatocyte growth factor
• UniProt Protein Name: Hepatocyte growth factor
• Protein Family: Hepatocyte growth factor
• UniProt Gene Name: Hgf
• UniProt Synonym Gene Names: SF
• UniProt Entry Name: HGF_MOUSE

NCBI Description

This gene encodes a protein that binds to the hepatocyte growth factor receptor to regulate cell growth, cell motility and morphogenesis in numerous cell and tissue types. The encoded preproprotein is proteolytically processed to generate multiple protein products, including the hepatocyte growth factor alpha and beta chains, which heterodimerize to form the mature active protein. Although this protein is a member of the peptidase S1 family of serine proteases, it lacks peptidase activity. Homozygous knockout mice for this gene exhibit embryonic lethality due to impaired development of the placenta and liver. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2015]

Uniprot Description

HGF: HGF is a potent mitogen for mature parenchymal hepatocyte cells, seems to be an hepatotrophic factor, and acts as growth factor for a broad spectrum of tissues and cell types. It has no detectable protease activity. Activating ligand for the receptor tyrosine kinase MET by binding and promoting its dimerization. Defects in HGF are the cause of deafness autosomal recessive type 39 (DFNB39). A form of profound prelingual sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information. Belongs to the peptidase S1 family. Plasminogen subfamily. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Hormone; Cell development/differentiation; Motility/polarity/chemotaxis

Cellular Component: extracellular space; membrane

Molecular Function: chemoattractant activity; growth factor activity; identical protein binding; protein heterodimerization activity; serine-type endopeptidase activity

Biological Process: activation of MAPK activity; cell morphogenesis; cell proliferation; hepatocyte growth factor receptor signaling pathway; liver development; myoblast proliferation; negative regulation of apoptosis; negative regulation of caspase activity; negative regulation of inflammatory response; negative regulation of interleukin-6 production; negative regulation of peptidyl-serine phosphorylation; positive chemotaxis; positive regulation of angiogenesis; positive regulation of cell migration; positive regulation of cell proliferation; positive regulation of interleukin-10 production; positive regulation of myelination; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of phosphoinositide 3-kinase cascade; proteolysis