COVID 19 Spike RBD Coronavirus Active Protein | COVID-19 active protein

Recombinant SARS-CoV Spike RBD protein

Cat. Num. AAA201279

• Applications: SDS-Page
• Purity: >95% by SDS-PAGE
• Synonyms: COVID 19 Spike RBD Coronavirus; Recombinant SARS-CoV Spike RBD protein; 2019 Novel Coronavirus; Coronavirus; CoV; COVID-19 virus; HCoV-2; Human Coronavirus 2019; SARS2; SARS-CoV-2; Severe acute respiratory syndrome coronavirus 2; Spike RBD; E2 glycoprotein precursor; Spike glycoprotein; S glycoprotein; E2; Peplomer protein; Severe acute repiratory Syndrome-related Coronavirus; SARS; SRAS-CoV; SARS-CoV1; COVID-19 active protein

• Host: HEK293
• Purity/Purification: >95% by SDS-PAGE
• Form/Format: Liquid. In Phosphate-Buffered Saline (pH7.4) containing 10% glycerol
• Concentration: 0.5 mg/ml (determined by Absorbance at 280nm) (varies by lot)
• Sequence Positions: 306-515aa
• Sequence: RVVPSG DVVRFPNITN LCPFGEVFNA TKFPSVYAWE RKKISNCVAD YSVLYNSTFF STFKCYGVSA TKLNDLCFSN VYADSFVVKG DDVRQIAPGQ TGVIADYNYK LPDDFMGCVL AWNTRNIDAT STGNYNYKYR YLRHGKLRPF ERDISNVPFS PDGKPCTPPA LNCYWPLNDY GFYTTTGIGY QPYRVVVLSF ELLNAPATVC GPKL

• Applicable Applications for COVID-19 active protein: SDS-PAGE, Bioactivity
• Endotoxin: <1 EU per 1ug of protein (determined by LAL method)
• Species: SARS-CoV Spike RBD
• Tag: His-Tag
• Predicted N-term: Asp
• Bioactivity: Measured by its binding ability in a functional ELISA with Human ACE-2
• Preparation and Storage: Can be stored at +2C to +8 degree C for 1 week. For long term storage, aliquot and store at -20C to -80 degree C. Avoid repeated freezing and thawing cycles.

SDS-Page

(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)

Biological Activity

(SARS-CoV Spike RBD is coated at 5ug/ml (100 ul/well) can bind ACE-2 (CAT# ATGP3963) in a Functional ELISA assay.)

Related Product Information for COVID-19 active protein

Severe Acute Respiratory Syndrome Coronavirus (SARS-CoV), Middle Eastern Respiratory Syndrome Coronavirus (MERS-CoV), and the recently identified novel Coronavirus (SARS-CoV-2) belong to the Coronaviridae family, genus Betacoronavirus, that has been related to important epidemiological outbreaks. SARS-CoV emerged in 2003 as a significant threat to human health. SARS-CoV has four structural proteins, known as the S (spike), E (envelope), M (membrane), and N (nucleocapsid) proteins. The spike protein, responsible for allowing the virus to attach to and fuse with the membrane of a host cell and is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion. The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. It attaches the virion to the cell membrane by interacting with host receptor, initiating the infection. A metallopeptidase, angiotensin-converting enzyme 2 (ACE-2), has been identified as a functional receptor for SARS-CoV through interaction with a receptor binding domain (RBD) located at the C-terminus of S1 subunit. Recombinant SARS-CoV spike RBD protein fused to His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.

Product Categories/Family for COVID-19 active protein

Virus; Proteins

References

Kukla M., et al, (2020) J Clin Med. 9:1420. Ayouba A., et al,(2020) J Clin Virol. 129:104521. Tortorici, M.A. and D. Veesler (2019). Adv. Virus Res. 105:93-116. Li F, et al, (2005) Science. 309:1864-1868. Struck AW, et al, (2012) Antiviral Res. 94:288-296.

NCBI and Uniprot Product Information

• UniProt Accession #: P59594
• Molecular Weight: 24.8kDa (220aa)
• UniProt Protein Name: Spike glycoprotein
• UniProt Gene Name: S
• UniProt Synonym Gene Names: S glycoprotein
• UniProt Entry Name: SPIKE_CVHSA

Uniprot Description

S1 attaches the virion to the cell membrane by interacting with human ACE2 and CLEC4M/DC-SIGNR, initiating the infection. Binding to the receptor and internalization of the virus into the endosomes of the host cell probably induces conformational changes in the S glycoprotein. Proteolysis by cathepsin CTSL may unmask the fusion peptide of S2 and activate membranes fusion within endosomes.