VEGF C Active Protein | VEGFC active protein

VEGF C Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8120343

• Gene Names: VEGFC; VRP; Flt4-L
• Purity: >95% as determined by SDS-PAGE
• Synonyms: VEGF C; VEGF C Protein; Human; Recombinant (His Tag); Human VEGF-C Protein (His Tag); Flt4-L Protein; LMPH1D Protein; VEGF-C Protein; VRP Protein; vascular endothelial growth factor C; VEGFC active protein

• Host: HEK293 Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Thr103-Arg227

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Thr 103
• Tag: C-His
• Protein Construction: A DNA sequence encoding the mature form of human VEGFC (NP_005420.1)(Thr103-Arg227) was expressed with a C-terminal polyhistidine tag.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Activity: 1. Measured by its binding ability in a functional ELISA. Immobilized VEGF C-his at 2ug/mL (100uL/well) can bind VEGFR3 hFc. The EC50 of VEGFR3 hFc is 2-15ng/mL.; 2. Scatchard analysis showed the affinity constant (Kd) of recombinant human VEGF-C bound to recombinant human VEGFR3 was 1.4nM.; 3. Measured in a cell proliferation assay using humanuMbilical vein endothelial cells (HUVEC). The ED50 for this effect is 0.05-0.2ug/mL.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Testing Data

(Measured by its binding ability in a functional ELISA. Immobilized VEGF C-his at 2 ug/mL (100 uL/well) can bind VEGFR3-His, Biotinylated, the EC50 of VEGFR3-His, Biotinylated is 1-10 ng/mL.)

Related Product Information for VEGFC active protein

Background: Vascular endothelial growth factor C (VEGF-C) is a member of the VEGF family. Upon biosynthesis, VEGF-C protein is secreted as a non-covalent momodimer in an anti-parellel fashion. VEGF-C protein is a dimeric glycoprotein, as a ligand for two receptors, VEGFR-3 (Flt4), and VEGFR-2. VEGF-C may function in angiogenesis of the venous and lymphatic vascular systems during embryogenesis. VEGF-C protein is over-expressed in various human cancers including breast cancer and prostate cancer. VEGF-C/VEGFR-3 axis, through different signaling pathways, plays a critical role in cancer progression by regulating different cellular functions, such as invasion, proliferation, and resistance to chemotherapy. Thus, targeting the VEGF-C/VEGFR-3 axis may be therapeutically significant for certain types of tumors.

Product Categories/Family for VEGFC active protein

Cancer Drug Targets

References

Joukov V, et al. (1997) Vascular endothelial growth factors VEGF-B and VEGF-C. J Cell Physiol. 173(2): 211-5.Su JL, et al. (2007) The role of the VEGF-C/VEGFR-3 axis in cancer progression. Br J Cancer. 96(4): 541-5.Anisimov A, et al. (2009) Activated forms of VEGF-C and VEGF-D provide improved vascular function in skeletal muscle. Circ Res. 104(11): 1302-12.

NCBI and Uniprot Product Information

• NCBI GI #: 4885653
• NCBI GeneID: 7424
• NCBI Accession #: NP_005420.1
• NCBI GenBank Nucleotide #: NM_005429.3
• UniProt Accession #: P49767; B2R9Q8
• Molecular Weight: 46,883 Da
• NCBI Official Full Name: vascular endothelial growth factor C preproprotein
• NCBI Official Synonym Full Names: vascular endothelial growth factor C
• NCBI Official Symbol: VEGFC
• NCBI Official Synonym Symbols: VRP; Flt4-L
• NCBI Protein Information: vascular endothelial growth factor C; VEGF-C; FLT4 ligand DHM; vascular endothelial growth factor-related protein
• UniProt Protein Name: Vascular endothelial growth factor C
• Protein Family: VEGF coregulated chemokine
• UniProt Gene Name: VEGFC
• UniProt Synonym Gene Names: VEGF-C; Flt4-L; VRP
• UniProt Entry Name: VEGFC_HUMAN

NCBI Description

The protein encoded by this gene is a member of the platelet-derived growth factor/vascular endothelial growth factor (PDGF/VEGF) family, is active in angiogenesis and endothelial cell growth, and can also affect the permeability of blood vessels. This secreted protein undergoes a complex proteolytic maturation, generating multiple processed forms which bind and activate VEGFR-3 receptors. Only the fully processed form can bind and activate VEGFR-2 receptors. This protein is structurally and functionally similar to vascular endothelial growth factor D. [provided by RefSeq, Jul 2008]

Uniprot Description

VEGFC: Growth factor active in angiogenesis, and endothelial cell growth, stimulating their proliferation and migration and also has effects on the permeability of blood vessels. May function in angiogenesis of the venous and lymphatic vascular systems during embryogenesis, and also in the maintenance of differentiated lymphatic endothelium in adults. Binds and activates VEGFR-2 (KDR/FLK1) and VEGFR-3 (FLT4) receptors. Homodimer; non-covalent and antiparallel. Spleen, lymph node, thymus, appendix, bone marrow, heart, placenta, ovary, skeletal muscle, prostate, testis, colon and small intestine and fetal liver, lung and kidney, but not in peripheral blood lymphocyte. Belongs to the PDGF/VEGF growth factor family.

Protein type: Motility/polarity/chemotaxis; Cell cycle regulation; Secreted, signal peptide; Secreted; Cytokine

Chromosomal Location of Human Ortholog: 4q34.3

Cellular Component: extracellular space; membrane; extracellular region

Molecular Function: protein binding; growth factor activity; vascular endothelial growth factor receptor 3 binding; chemoattractant activity

Biological Process: response to drug; substrate-bound cell migration; platelet activation; positive regulation of neuroblast proliferation; positive regulation of blood vessel endothelial cell migration; signal transduction; positive regulation of protein amino acid autophosphorylation; induction of positive chemotaxis; negative regulation of cell proliferation; organ morphogenesis; positive regulation of angiogenesis; positive regulation of peptidyl-tyrosine phosphorylation; morphogenesis of embryonic epithelium; platelet degranulation; positive regulation of protein secretion; positive chemotaxis; positive regulation of cell division; regulation of vascular endothelial growth factor receptor signaling pathway; positive regulation of cell proliferation; angiogenesis; negative regulation of blood pressure; vascular endothelial growth factor receptor signaling pathway; positive regulation of cell-matrix adhesion; blood coagulation; positive regulation of epithelial cell proliferation

Disease: Lymphedema, Hereditary, Id