Human VEGF-C ELISA Kit | VEGF-C elisa kit

VEGF-C Human ELISA Kit

Cat. Num. AAA355372

• Gene Names: VEGFC; VRP; Flt4-L
• Reactivity: Human
• Applications: ELISA
• Synonyms: VEGF-C; VEGF-C Human ELISA Kit; Human VEGF-C; VEGF-C elisa kit

• Reactivity: Human

• Applicable Applications for VEGF-C elisa kit: Quantitative sELISA (EIA)
• Application Notes: For quantitative detection of VEGF-C in human serum, plasma, body fluids, tissue lysates or cell culture supernatants.
• Assay Type: Sandwich
• Detection Range: 31.2 pg/ml-2000 pg/ml (human serum, body fluids)
• Sensitivity: < 3 pg/ml
• Preparation and Storage: Store at 2-8 degree C for 4 months, or at -20 degree C for 8 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for VEGF-C elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-VEGF-C polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-VEGF-C polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the VEGF-C amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of VEGF-C can be calculated.

Background: Vascular endothelial growth factor C (VEGF-C) is a member of the platelet-derived growth factor/vascular endothelial growth factor (PDGF/VEGF) family. It is active in angiogenesis, lymphangiogenesis and endothelial cell growth and survival, and can also affect the permeability of blood vessels. This secreted protein undergoes a complex proteolytic maturation, generating multiple processed forms that bind and activate VEGFR-3 receptors. Karkkainen et al. (2004) found that VEGFC and VEGF, unlike VEGFB and VEGFD, are essential for embryonic survival and lymphangiogenesis.

NCBI and Uniprot Product Information

• NCBI GI #: 4885653
• NCBI GeneID: 7424
• NCBI Accession #: NP_005420.1
• NCBI GenBank Nucleotide #: NM_005429.3
• UniProt Accession #: P49767; B2R9Q8
• Molecular Weight: 46,883 Da
• NCBI Official Full Name: vascular endothelial growth factor C preproprotein
• NCBI Official Synonym Full Names: vascular endothelial growth factor C
• NCBI Official Symbol: VEGFC
• NCBI Official Synonym Symbols: VRP; Flt4-L
• NCBI Protein Information: vascular endothelial growth factor C; VEGF-C; FLT4 ligand DHM; vascular endothelial growth factor-related protein
• UniProt Protein Name: Vascular endothelial growth factor C
• UniProt Gene Name: VEGFC
• UniProt Synonym Gene Names: VEGF-C; Flt4-L; VRP
• UniProt Entry Name: VEGFC_HUMAN

NCBI Description

The protein encoded by this gene is a member of the platelet-derived growth factor/vascular endothelial growth factor (PDGF/VEGF) family, is active in angiogenesis and endothelial cell growth, and can also affect the permeability of blood vessels. This secreted protein undergoes a complex proteolytic maturation, generating multiple processed forms which bind and activate VEGFR-3 receptors. Only the fully processed form can bind and activate VEGFR-2 receptors. This protein is structurally and functionally similar to vascular endothelial growth factor D. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Growth factor active in angiogenesis, and endothelial cell growth, stimulating their proliferation and migration and also has effects on the permeability of blood vessels. May function in angiogenesis of the venous and lymphatic vascular systems during embryogenesis, and also in the maintenance of differentiated lymphatic endothelium in adults. Binds and activates VEGFR-2 (KDR/FLK1) and VEGFR-3 (FLT4) receptors. Ref.11

Subunit structure: Homodimer; non-covalent and antiparallel. Ref.11

Subcellular location: Secreted.

Tissue specificity: Spleen, lymph node, thymus, appendix, bone marrow, heart, placenta, ovary, skeletal muscle, prostate, testis, colon and small intestine and fetal liver, lung and kidney, but not in peripheral blood lymphocyte.

Post-translational modification: Undergoes a complex proteolytic maturation which generates a variety of processed secreted forms with increased activity toward VEGFR-3, but only the fully processed form could activate VEGFR-2. VEGF-C first form an antiparallel homodimer linked by disulfide bonds. Before secretion, a cleavage occurs between Arg-227 and Ser-228 producing a heterotetramer. The next extracellular step of the processing removes the N-terminal propeptide. Finally the mature VEGF-C is composed mostly of two VEGF homology domains (VHDs) bound by non-covalent interactions.

Sequence similarities: Belongs to the PDGF/VEGF growth factor family.