DcR1/TRAILR3 Cell Lysate | TNFRSF10C cell lysate
Human DcR1/TRAILR3 HEK293 Overexpression Lysate
Gene Names
TNFRSF10C; LIT; DCR1; TRID; CD263; TRAILR3; TRAIL-R3; DCR1-TNFR
Applications
Western Blot
Synonyms
DcR1/TRAILR3; Human DcR1/TRAILR3 HEK293 Overexpression Lysate; Human TRAILR3/TNFRSF10C HEK293 Cell Lysate (WB positive control); tumor necrosis factor receptor superfamily member 10c; Human CD263 Overexpression Lysate; Human DCR1 Overexpression Lysate; Human DCR1-TNFR Overexpression Lysate; Human LIT Overexpression Lysate; Human TRAIL-R3 Overexpression Lysate; Human TRAILR3 Overexpression Lysate; Human TRID Overexpression Lysate; TNFRSF10C cell lysate
Host
HEK293 Cells
Form/Format
1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Sequence Positions
221aa
Sequence
Met1-Pro235
Applicable Applications for TNFRSF10C cell lysate
Western Blot (WB)
Application Notes
WB: Use at an assay dependent dilution.
Species
Human
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
Sequence Construction
A DNA sequence encoding the human TNFRSF10C (NP_003832.2) (Met1-Pro235) was expressed with a polyhistidine tag at the C-terminus.
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Preparation and Storage
Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.
Samples are stable for up to twelve months from date of receipt.
Related Product Information for TNFRSF10C cell lysate
TNFRSF10C CNV in patients with CRC is associated with distant metastatic disease. A high frequency of CGI methylation in the TNFRSF10C promoter results in inactivation of the gene and enhancement of tumor growth in most PC cell lines (except CFPAC-1). Inactivation of TNFRSF10C by CpG island (CGI) hypermethylation can play an important role in PC progression and be potentially useful as a marker and a new therapeutic approach for PC.
This Human DcR1/TRAILR3 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of DcR1/TRAILR3 protein from the overexpression lysate was verified.
This Human DcR1/TRAILR3 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of DcR1/TRAILR3 protein from the overexpression lysate was verified.
Product Categories/Family for TNFRSF10C cell lysate
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
27,407 Da
NCBI Official Full Name
tumor necrosis factor receptor superfamily member 10C
NCBI Official Synonym Full Names
tumor necrosis factor receptor superfamily member 10c
NCBI Official Symbol
TNFRSF10C
NCBI Official Synonym Symbols
LIT; DCR1; TRID; CD263; TRAILR3; TRAIL-R3; DCR1-TNFR
NCBI Protein Information
tumor necrosis factor receptor superfamily member 10C
UniProt Protein Name
Tumor necrosis factor receptor superfamily member 10C
Protein Family
UniProt Gene Name
TNFRSF10C
UniProt Synonym Gene Names
DCR1; LIT; TRAILR3; TRID; DcR1; TRAIL receptor 3; TRAIL-R3
UniProt Entry Name
TR10C_HUMAN
Uniprot Description
TRAIL-R3: Receptor for the cytotoxic ligand TRAIL. Lacks a cytoplasmic death domain and hence is not capable of inducing apoptosis. May protect cells against TRAIL mediated apoptosis by competing with TRAIL-R1 and R2 for binding to the ligand. Higher expression in normal tissues than in tumor cell lines. Highly expressed in peripheral blood lymphocytes, spleen, skeletal muscle, placenta, lung and heart.
Protein type: Membrane protein, GPI anchor
Chromosomal Location of Human Ortholog: 8p22-p21
Cellular Component: integral to plasma membrane
Molecular Function: transmembrane receptor activity; TRAIL binding
Biological Process: apoptosis; signal transduction
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Product Notes
The TNFRSF10C tnfrsf10c (Catalog #AAA8113835) is a Cell Lysate produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 221aa. AAA Biotech's DcR1/TRAILR3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: Use at an assay dependent dilution. Researchers should empirically determine the suitability of the TNFRSF10C tnfrsf10c for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Met1-Pro23 5. It is sometimes possible for the material contained within the vial of "DcR1/TRAILR3, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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