Tomoregulin-1 Cell Lysate | TMEFF1 cell lysate
Human Tomoregulin-1 HEK293 Overexpression Lysate
Gene Names
TMEFF1; TR-1; H7365; C9orf2; CT120.1
Applications
Western Blot
Synonyms
Tomoregulin-1; Human Tomoregulin-1 HEK293 Overexpression Lysate; Human TMEFF1/Tomoregulin-1 (ECD) HEK293 Cell Lysate (WB positive control); transmembrane protein with EGF-like and two follistatin-like domains 1; Human C9orf2 Overexpression Lysate; Human CT120.1 Overexpression Lysate; Human H7365 Overexpression Lysate; Human TR-1 Overexpression Lysate; TMEFF1 cell lysate
Host
HEK293 Cells
Form/Format
1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Sequence Positions
532aa
Sequence
Met1-Val330
Applicable Applications for TMEFF1 cell lysate
Western Blot (WB)
Application Notes
WB: Use at an assay dependent dilution.
Species
Human
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
Sequence Construction
A DNA sequence encoding the human TMEFF1 (NP_003683.2) (Met1-Val330) was expressed with the Fc region of human IgG1 at the C-terminus.
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Preparation and Storage
Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.
Samples are stable for up to twelve months from date of receipt.
Related Product Information for TMEFF1 cell lysate
TMEFF1 is a novel transmembrane protein, containing two follisatin domains and an epidermal growth factor-like region. The ectopic expression of TMEFF1 in brain cancer cells resulted in their growth inhibition. That TMEFF1 may behave as a tumor suppressor gene in brain cancers. TMEFF1 and TMEFF2 are putative transmembrane proteins comprised of one epidermal growth factor (EGF)-like domain and two follistatin-like domains. Both TMEFF1 and TMEFF2 are predominantly expressed in the brain.
This Human Tomoregulin-1 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of Tomoregulin-1 protein from the overexpression lysate was verified.
This Human Tomoregulin-1 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of Tomoregulin-1 protein from the overexpression lysate was verified.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
37,757 Da
NCBI Official Full Name
tomoregulin-1
NCBI Official Synonym Full Names
transmembrane protein with EGF like and two follistatin like domains 1
NCBI Official Symbol
TMEFF1
NCBI Official Synonym Symbols
TR-1; H7365; C9orf2; CT120.1
NCBI Protein Information
tomoregulin-1
UniProt Protein Name
Tomoregulin-1
Protein Family
UniProt Gene Name
TMEFF1
UniProt Synonym Gene Names
C9orf2; TR-1
UniProt Entry Name
TEFF1_HUMAN
Uniprot Description
TMEFF1: a single-pass type I membrane protein. May inhibit NODAL and BMP signaling during neural patterning. A member of the Cancer-Testis Antigen (CTA) superfamily. CTAs may play roles in embryonal development and tumor transformation or aspects of tumor progression. CTAs were once thought to be silenced in most normal adult tissues, with limited expression in fetal, placental, testis, and ovarian cells. These proteins are now known to be aberrantly expressed in various cancers and many are capable of eliciting humoral and cellular immune responses. Expressed predominantly in brain, and at lower levels in heart, placenta and skeletal muscle. Down-regulated in brain tumors as compared to control brain tissues. Expressed in a variety of cancers cell lines and in myeloma cells. Belongs to the tomoregulin family. Two isoforms of the human protein are produced by alternative splicing. Note: This description may include information from RefSeq and UniProtKB
Protein type: Cancer Testis Antigen (CTA); Membrane protein, integral
Chromosomal Location of Human Ortholog: 9q31
Cellular Component: plasma membrane; integral to membrane
Biological Process: multicellular organismal development
Research Articles on TMEFF1
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Product Notes
The TMEFF1 tmeff1 (Catalog #AAA8114289) is a Cell Lysate produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 532aa. AAA Biotech's Tomoregulin-1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: Use at an assay dependent dilution. Researchers should empirically determine the suitability of the TMEFF1 tmeff1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Met1-Val33 0. It is sometimes possible for the material contained within the vial of "Tomoregulin-1, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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