TIE2 Cell Lysate | TEK cell lysate
Mouse TIE2 HEK293 Overexpression Lysate
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Samples are stable for up to twelve months from date of receipt.
This Mouse TIE2 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of TIE2 protein from the overexpression lysate was verified.
NCBI and Uniprot Product Information
Uniprot Description
TIE2: a receptor tyrosine kinase of the Tie family. Receptor for angiopoietin 1. Expressed almost exclusively in endothelial cells. May constitute the earliest mammalian endothelial cell lineage marker. Appears to be critical for endothelial cell-smooth muscle cell communication in venous morphogenesis. TEK is closely related to the TIE receptor tyrosine kinase.
Protein type: EC 2.7.10.1; Kinase, protein; Membrane protein, integral; Protein kinase, TK; Protein kinase, tyrosine (receptor); TK group; Tie family
Cellular Component: actin filament; apical plasma membrane; basal plasma membrane; basolateral plasma membrane; cell surface; integral to membrane; integral to plasma membrane; intercellular junction; lipid raft; microvillus; perinuclear region of cytoplasm; stress fiber
Molecular Function: growth factor binding; protein binding; protein-tyrosine kinase activity; receptor activity
Biological Process: angiogenesis; cell-cell adhesion; cell-matrix adhesion; endothelial cell proliferation; heart development; hemopoiesis; negative regulation of angiogenesis; negative regulation of apoptosis; patterning of blood vessels; peptidyl-tyrosine phosphorylation; positive regulation of angiogenesis; positive regulation of cell adhesion; positive regulation of cytokine secretion during immune response; positive regulation of focal adhesion formation; positive regulation of peptidyl-serine phosphorylation; positive regulation of phosphoinositide 3-kinase activity; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein amino acid phosphorylation; positive regulation of protein import into nucleus; positive regulation of protein kinase B signaling cascade; positive regulation of vascular endothelial growth factor receptor signaling pathway; protein amino acid autophosphorylation; protein oligomerization; regulation of angiogenesis; regulation of cell migration; regulation of endothelial cell proliferation; regulation of establishment and/or maintenance of cell polarity; response to estrogen stimulus; response to retinoic acid; sprouting angiogenesis; Tie receptor signaling pathway; transmembrane receptor protein tyrosine kinase signaling pathway; vasculogenesis
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Product Notes
The TEK tek (Catalog #AAA8117025) is a Cell Lysate produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 737aa. AAA Biotech's TIE2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: Use at an assay dependent dilution. Researchers should empirically determine the suitability of the TEK tek for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Met1-Lys74 4. It is sometimes possible for the material contained within the vial of "TIE2, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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