Guinea Pig C4BPalpha ELISA Kit | C4BPalpha elisa kit
Guinea pig C4BPalpha (C4 Binding Protein Alpha) ELISA Kit
Reactivity
Guinea Pig
Synonyms
C4BPalpha; Guinea pig C4BPalpha (C4 Binding Protein Alpha) ELISA Kit; C4BPalpha elisa kit
Reactivity
Guinea Pig
Specificity
This kit recognizes natural and recombinant Guinea pig C4BPalpha. No significant cross-reactivity or interference between Guinea pig C4BPalpha and analogues was observed.
Samples
Serum, Plasma, Biological Fluids
Assay Type
Sandwich
Detection Range
62.5-4000pg/mL
Sensitivity
Min: 37.5pg/mL; Max: 4000pg/mL
Preparation and Storage
Store at 4 degree C.
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for C4BPalpha elisa kit
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Guinea pig C4BPalpha concentrations in serum, plasma and other biological fluids.
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to C4BPalpha. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for C4BPalpha and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain C4BPalpha, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of C4BPalpha. You can calculate the concentration of C4BPalpha in the samples by comparing the OD of the samples to the standard curve.
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to C4BPalpha. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for C4BPalpha and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain C4BPalpha, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of C4BPalpha. You can calculate the concentration of C4BPalpha in the samples by comparing the OD of the samples to the standard curve.
