Tie2 / TEK recombinant protein

Recombinant Mouse Tie2 / TEK Protein (aa 770-1122, His & GST tag)

Cat. Num. AAA2546672

• Gene Names: Tek; Hyk; STK1; Tie2; Tie-2; Cd202b; AA517024
• Purity: > 91 % as determined by SDS-PAGE
• Synonyms: Tie2 / TEK; Recombinant Mouse Tie2 / TEK Protein (aa 770-1122; His & GST tag); TEK; Hyk; Tie-2; Tie2; Tie2 / TEK recombinant protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: > 91 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 8.0, 10% gly
• Sequence Length: 1122

• Application Notes: The recombinant mouse TEK/GST chimera consists of 590 amino acids and has a calculated molecular mass of 68.2kDa. The recombinant protein migrates as an approximately 68 kDa band in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Met
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for Tie2 / TEK recombinant protein

Background: TEK, or TIE-2, is an endothelial cell-specific receptor tyrosine kinase (RTK) that is known as a functioning molecule of vascular endothelial cells. TEK comprises a subfamily of RTK with TIE, and these two receptors play critical roles in vascular maturation, maintenance of integrity and remodeling. Targeted mutagenesis of both Tek and its agonistic ligand, Angiopoietin-1, result in embryonic lethality, demonstrating that the signal transduction pathways mediated by this receptor are crucial for normal embryonic development. TEK signaling is indispensable for the development of the embryonic vasculature and suggests that TEK signaling may also be required for the development of the tumor vasculature.

Description: A DNA sequence encoding the mouse TEK (Q02858) (Gln770-Ala1122) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 594542525
• NCBI GeneID: 21687
• NCBI Accession #: NP_001277478.1
• NCBI GenBank Nucleotide #: NM_001290549.1
• UniProt Accession #: Q02858
• Molecular Weight: 125,701 Da
• NCBI Official Full Name: angiopoietin-1 receptor isoform 2
• NCBI Official Synonym Full Names: endothelial-specific receptor tyrosine kinase
• NCBI Official Symbol: Tek
• NCBI Official Synonym Symbols: Hyk; STK1; Tie2; Tie-2; Cd202b; AA517024
• NCBI Protein Information: angiopoietin-1 receptor
• UniProt Protein Name: Angiopoietin-1 receptor
• UniProt Gene Name: Tek
• UniProt Synonym Gene Names: Hyk; Tie-2; Tie2; mTIE2

Uniprot Description

TIE2: a receptor tyrosine kinase of the Tie family. Receptor for angiopoietin 1. Expressed almost exclusively in endothelial cells. May constitute the earliest mammalian endothelial cell lineage marker. Appears to be critical for endothelial cell-smooth muscle cell communication in venous morphogenesis. TEK is closely related to the TIE receptor tyrosine kinase.

Protein type: EC 2.7.10.1; Kinase, protein; Membrane protein, integral; Protein kinase, TK; Protein kinase, tyrosine (receptor); TK group; Tie family

Chromosomal Location of Human Ortholog: 4 C5|4 43.34 cM

Cellular Component: actin filament; apical plasma membrane; basal plasma membrane; basolateral plasma membrane; cell surface; integral to membrane; integral to plasma membrane; intercellular junction; lipid raft; microvillus; perinuclear region of cytoplasm; stress fiber

Molecular Function: growth factor binding; protein binding; protein-tyrosine kinase activity; receptor activity

Biological Process: angiogenesis; cell-cell adhesion; cell-matrix adhesion; endothelial cell proliferation; heart development; hemopoiesis; negative regulation of angiogenesis; negative regulation of apoptosis; patterning of blood vessels; peptidyl-tyrosine phosphorylation; positive regulation of angiogenesis; positive regulation of cell adhesion; positive regulation of cytokine secretion during immune response; positive regulation of focal adhesion formation; positive regulation of peptidyl-serine phosphorylation; positive regulation of phosphoinositide 3-kinase activity; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein amino acid phosphorylation; positive regulation of protein import into nucleus; positive regulation of protein kinase B signaling cascade; positive regulation of vascular endothelial growth factor receptor signaling pathway; protein amino acid autophosphorylation; protein oligomerization; regulation of angiogenesis; regulation of cell migration; regulation of endothelial cell proliferation; regulation of establishment and/or maintenance of cell polarity; response to estrogen stimulus; response to retinoic acid; sprouting angiogenesis; Tie receptor signaling pathway; transmembrane receptor protein tyrosine kinase signaling pathway; vasculogenesis