Neuroserpin Cell Lysate | SerpinI1 cell lysate
Mouse Neuroserpin HEK293 Overexpression Lysate
Gene Names
Serpini1; Ns; PI12; PI-12; Spi17; AI837402
Applications
Western Blot
Synonyms
Neuroserpin; Mouse Neuroserpin HEK293 Overexpression Lysate; Mouse SerpinI1/Neuroserpin HEK293 Cell Lysate (WB positive control); serpin peptidase inhibitor; clade I (neuroserpin); member 1; Mouse AI837402 Overexpression Lysate; Mouse Ns Overexpression Lysate; Mouse PI-12 Overexpression Lysate; Mouse PI12 Overexpression Lysate; Mouse Serpin I1 Overexpression Lysate; Mouse Spi17 Overexpression Lysate; SerpinI1 cell lysate
Host
HEK293 Cells
Form/Format
1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Sequence Positions
405aa
Sequence
Met1-Leu410
Applicable Applications for SerpinI1 cell lysate
Western Blot (WB)
Application Notes
WB: Use at an assay dependent dilution.
Species
Mouse
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
Sequence Construction
A DNA sequence encoding the mouse SERPINI1 (O35684) (Met 1-Leu 410) was expressed, with a C-terminal polyhistidine tag.
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Preparation and Storage
Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.
Samples are stable for up to twelve months from date of receipt.
Related Product Information for SerpinI1 cell lysate
Neuroserpin, also known as Protease inhibitor 12 and SERPINI1, is a secreted protein that belongs to the serpin family. Neuroserpin is a serine protease inhibitor that inhibits plasminogen activators and plasmin but not thrombin. Serine protease inhibitors of the serpin superfamily are involved in many cellular processes. Neuroserpin was first identified as a protein secreted from the axons of dorsal root ganglion neurons. Neuroserpin is predominantly expressed in the brain, and is expressed in the late stages of neurogenesis during the process of synapse formation. Overexpression of neuroserpin in an anterior pituitary corticotroph cell line results in the extension of neurite-like processes, suggesting that neuroserpin may play a role in cell communication, cell adhesion, and/or cell migration. Neuroserpin may be involved in the formation or reorganization of synaptic connections, as well as synaptic plasticity in the adult nervous system. Neuroserpin may also protect neurons from cell damage by tissue-type plasminogen activator. Defects of neuroserpin are the cause of familial encephalopathy with neuroserpin inclusion bodies (FEN1B).
This Mouse Neuroserpin overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of Neuroserpin protein from the overexpression lysate was verified.
This Mouse Neuroserpin overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of Neuroserpin protein from the overexpression lysate was verified.
References
Schrimpf SP. et al., 1997, Genomics. 40 (1): 55-62. Hill RM. et al., 2002, Ann N Y Acad Sci. 971: 406-15. Yepes M. et al., 2004, Thromb. Haemost. 91 (3): 457-64. Galliciotti G. et al., 2006, Front Biosci. 11: 33-45.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
160,000
NCBI Official Full Name
neuroserpin
NCBI Official Synonym Full Names
serine (or cysteine) peptidase inhibitor, clade I, member 1
NCBI Official Symbol
Serpini1
NCBI Official Synonym Symbols
Ns; PI12; PI-12; Spi17; AI837402
NCBI Protein Information
neuroserpin; serpin I1; protease inhibitor 17; peptidase inhibitor 12; serine protease inhibitor 17
UniProt Protein Name
Neuroserpin
Protein Family
UniProt Gene Name
Serpini1
UniProt Synonym Gene Names
Pi12; Spi17; PI-12
UniProt Entry Name
NEUS_MOUSE
Uniprot Description
SERPINI1: Serine protease inhibitor that inhibits plasminogen activators and plasmin but not thrombin. May be involved in the formation or reorganization of synaptic connections as well as for synaptic plasticity in the adult nervous system. May protect neurons from cell damage by tissue-type plasminogen activator. Defects in SERPINI1 are the cause of familial encephalopathy with neuroserpin inclusion bodies (FEN1B). FEN1B is characterized clinically as an autosomal dominantly inherited dementia, histologically by unique neuronal inclusion bodies and biochemically by polymers of neuroserpin. Belongs to the serpin family.
Protein type: Secreted; Secreted, signal peptide
Cellular Component: extracellular space; extracellular region
Molecular Function: serine-type endopeptidase inhibitor activity; protease inhibitor activity
Biological Process: regulation of cell adhesion; negative regulation of peptidase activity
Research Articles on SerpinI1
Similar Products
Product Notes
The SerpinI1 serpini1 (Catalog #AAA8116933) is a Cell Lysate produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 405aa. AAA Biotech's Neuroserpin can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: Use at an assay dependent dilution. Researchers should empirically determine the suitability of the SerpinI1 serpini1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Met1-Leu41 0. It is sometimes possible for the material contained within the vial of "Neuroserpin, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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