CD31 Active Protein | PECAM1 active protein

CD31 Protein, Human, Recombinant

Cat. Num. AAA8120026

• Gene Names: PECAM1; CD31; PECA1; GPIIA'; PECAM-1; endoCAM; CD31/EndoCAM
• Purity: >95% as determined by SDS-PAGE
• Synonyms: CD31; CD31 Protein; Human; Recombinant; Human CD31/PECAM1 Protein; CD31/EndoCAM Protein; endoCAM Protein; GPIIA' Protein; PECA1 Protein; PECAM-1 Protein; platelet/endothelial cell adhesion molecule 1; PECAM1 active protein

• Host: HEK293 Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 100mM Glycine, 10mM NaCl, 50mM Tris, pH7.5. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Lys601

• Species: Human
• Activity: Measured by the ability of the immobilized protein to support the adhesion of Jurkat human acute T cell leukemia cells. When 8×104cells/well are added to CD31-coated plates (5ug/mL, 100uL/well) in the presence of 10ug/mL PHA,approximately >60% will adhere after 30 minutes at 37 degree C.
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Gln 28
• Tag: C-cleavage
• Protein Construction: The mature form of human CD31 (EAW94208.1) extracellular domain (Met1-Lys 601) with five additional amino acids (DDDDK) at the C-terminus was expressed and purified.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for PECAM1 active protein

Background: The Cluster of Differentiation 31 (CD31) adhesion molecule, also known as platelet-endothelial cell adhesion molecule-1 (PECAM-1), is the only known member of the CAM family on platelets. CD31 protein is a 130-kDa transmembrane glycoprotein expressed by endothelial cells, platelets, monocytes, neutrophils, and certain T cell subsets. CD31 protein is also expressed in certain tumors, including epithelioid hemangioendothelioma, other vascular tumors, and histiocytic malignancies. CD31 plays a key role in removing aged neutrophils and tissue regeneration. CD31 protein mediates the homotypic or heterotypic cell adhesion by binding to itself or the leukocyte integrin alphavbeta3, and thus plays a role in neutrophil recruitment in inflammatory responses, transendothelial migration of leukocytes, as well as in cardiovascular development.

Product Categories/Family for PECAM1 active protein

Hemangioblast Markers ITIM/ITAM Immunoreceptors and Related Molecules

References

Deaglio S, et al. (2000) CD38/CD31, a receptor/ligand system ruling adhesion and signaling in human leukocytes. Chem Immunol. 75: 99-120.Kohler S, et al. (2009) Life after the thymus: CD31+ and CD31- human naive CD4+ T-cell subsets. Blood. 113(4): 769-74.

NCBI and Uniprot Product Information

• NCBI GI #: 119614614
• NCBI GeneID: 5175
• Molecular Weight: 81,378 Da
• NCBI Official Full Name: platelet/endothelial cell adhesion molecule (CD31 antigen), isoform CRA_a
• NCBI Official Synonym Full Names: platelet and endothelial cell adhesion molecule 1
• NCBI Official Symbol: PECAM1
• NCBI Official Synonym Symbols: CD31; PECA1; GPIIA'; PECAM-1; endoCAM; CD31/EndoCAM
• NCBI Protein Information: platelet endothelial cell adhesion molecule
• Protein Family: Platelet endothelial cell adhesion molecule

NCBI Description

The protein encoded by this gene is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. The encoded protein is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation. [provided by RefSeq, May 2010]