Human Protein Wnt-5a ELISA Kit | WNT5A elisa kit

Human Protein Wnt-5a ELISA Kit

Cat. Num. AAA763113

• Gene Names: WNT5A; hWNT5A
• Reactivity: Human
• Synonyms: Protein Wnt-5a; Human Protein Wnt-5a ELISA Kit; WNT5A/hWNT5A (Protein Wnt-5a)/wingless-type MMTV integration site family; member 5A/WNT-5A protein; WNT5A elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of WNT5A. No significant cross-reactivity or interference between WNT5A and analogues was observed.
• Sequence Length: 365

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for WNT5A elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 371502087
• NCBI GeneID: 7474
• NCBI Accession #: NP_001243034.1
• NCBI GenBank Nucleotide #: NM_001256105.1
• Molecular Weight: 40,887 Da
• NCBI Official Full Name: protein Wnt-5a isoform 2
• NCBI Official Synonym Full Names: wingless-type MMTV integration site family member 5A
• NCBI Official Symbol: WNT5A
• NCBI Official Synonym Symbols: hWNT5A
• NCBI Protein Information: protein Wnt-5a
• UniProt Protein Name: Protein Wnt-5a
• Protein Family: Protein
• UniProt Gene Name: WNT5A
• UniProt Entry Name: WNT5A_HUMAN

NCBI Description

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene encodes a member of the WNT family that signals through both the canonical and non-canonical WNT pathways. This protein is a ligand for the seven transmembrane receptor frizzled-5 and the tyrosine kinase orphan receptor 2. This protein plays an essential role in regulating developmental pathways during embryogenesis. This protein may also play a role in oncogenesis. Mutations in this gene are the cause of autosomal dominant Robinow syndrome. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2012]

Uniprot Description

WNT5A: Ligand for members of the frizzled family of seven transmembrane receptors. Can activate or inhibit canonical Wnt signaling, depending on receptor context. In the presence of FZD4, activates beta-catenin signaling. In the presence of ROR2, inhibits the canonical Wnt pathway by promoting beta-catenin degradation through a GSK3-independent pathway which involves down-regulation of beta-catenin-induced reporter gene expression. Suppression of the canonical pathway allows chondrogenesis to occur and inhibits tumor formation. Stimulates cell migration. Decreases proliferation, migration, invasiveness and clonogenicity of carcinoma cells and may act as a tumor suppressor. Mediates motility of melanoma cells. Required during embryogenesis for extension of the primary anterior-posterior axis and for outgrowth of limbs and the genital tubercle. Inhibits type II collagen expression in chondrocytes. Interacts with PORCN. Interacts with WLS. Expression is increased in differentiated thyroid carcinomas compared to normal thyroid tissue and anaplastic thyroid tumors where expression is low or undetectable. Expression is found in thyrocytes but not in stromal cells. Belongs to the Wnt family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 3p21-p14

Cellular Component: cell surface; endoplasmic reticulum lumen; extracellular region; extracellular space; Golgi lumen; plasma membrane; proteinaceous extracellular matrix

Molecular Function: cytokine activity; frizzled binding; frizzled-2 binding; protein binding; protein domain specific binding; receptor agonist activity; receptor tyrosine kinase-like orphan receptor binding; transcription factor activity

Biological Process: activation of JNK activity; activation of MAPK activity; activation of NF-kappaB transcription factor; activation of protein kinase B; alveolus development; axon guidance; cartilage development; cell fate commitment; cervix development; convergent extension involved in axis elongation; convergent extension involved in organogenesis; determination of anterior/posterior axis, embryo; dorsal/ventral pattern formation; embryonic digit morphogenesis; embryonic skeletal development; epithelial to mesenchymal transition; establishment of epithelial cell polarity; establishment of planar polarity; genitalia development; heart looping; hindgut morphogenesis; hypophysis morphogenesis; keratinocyte differentiation; lens development in camera-type eye; male gonad development; midbrain development; midgut development; negative chemotaxis; negative regulation of apoptosis; negative regulation of axon extension involved in axon guidance; negative regulation of BMP signaling pathway; negative regulation of epithelial cell proliferation; negative regulation of fat cell differentiation; negative regulation of fibroblast growth factor receptor signaling pathway; negative regulation of melanin biosynthetic process; negative regulation of synaptogenesis; negative regulation of transcription, DNA-dependent; neuron differentiation; notochord morphogenesis; olfactory bulb interneuron development; palate development; paraxial mesoderm formation; positive regulation of angiogenesis; positive regulation of cGMP metabolic process; positive regulation of chemokine biosynthetic process; positive regulation of cytokine secretion during immune response; positive regulation of endocytosis; positive regulation of endothelial cell proliferation; positive regulation of fibroblast proliferation; positive regulation of inflammatory response; positive regulation of interferon-gamma production; positive regulation of interleukin-1 beta secretion; positive regulation of interleukin-6 production; positive regulation of macrophage activation; positive regulation of meiosis; positive regulation of mesenchymal cell proliferation; positive regulation of ossification; positive regulation of peptidyl-serine phosphorylation; positive regulation of protein catabolic process; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; prostate gland development; response to estradiol stimulus; response to glucocorticoid stimulus; response to hyperoxia; response to organic substance; response to testosterone stimulus; somitogenesis; synaptogenesis; urinary bladder development; uterus development; vagina development; Wnt receptor signaling pathway; Wnt receptor signaling pathway through beta-catenin; Wnt receptor signaling pathway, calcium modulating pathway; Wnt receptor signaling pathway, planar cell polarity pathway; wound healing

Disease: Robinow Syndrome, Autosomal Dominant