PDGF-C Active Protein | PDGFC active protein

PDGF-C Protein, Human, Recombinant (hFc Tag)

Cat. Num. AAA8120154

• Gene Names: PDGFC; SCDGF; FALLOTEIN
• Purity: >97% as determined by SDS-PAGE
• Synonyms: PDGF-C; PDGF-C Protein; Human; Recombinant (hFc Tag); Human PDGF-C Protein (Fc Tag); FALLOTEIN Protein; SCDGF Protein; platelet derived growth factor C; PDGFC active protein

• Host: HEK293 Cells
• Purity/Purification: >97% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 100mM Glycine, 10mM NaCl, 50mM Tris, pH7.5. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Val235-Gly345

• Species: Human
• Activity: Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. The ED50 for this effect is typically 0.5-3ug/mL.
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Glu 20
• Tag: N-human IgG1-Fc
• Protein Construction: A DNA sequence encoding the mature form of human PDGF-C (NP_057289.1)(Val 235-Gly 345) was expressed with the fused Fc region of human IgG1 at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for PDGFC active protein

Background: PDGF-C is a member of the PDGF/VEGF family of growth factors with a unique domain organization and expression pattern. Platelet-derived growth factor receptors (PDGFRs) are catalytic receptors that have intracellular tyrosine kinase activity. They have roles in the regulation of many biological processes including embryonic development, angiogenesis, cell proliferation and differentiation, and contribute to the pathophysiology of some diseases, including cancer. There are two isoforms of the PDGFR receptor; PDGFRalpha and PDGFRbeta, which can form homo- or heterodimers. The endogenous PDGFR ligands are PDGF-A, -B, -C and -D, which induce receptor dimerization and transphosphorylation at specific tyrosine residues upon binding. This activates the intracellular kinase activity, initiating intracellular signaling through the MAPK, PI 3-K and PKCgamma pathways. PDGF-C acts as a specific ligand for alpha platelet-derived growth factor receptor homodimer, and alpha and beta heterodimer. Binding of this growth factor to its affinity receptor elicits a variety of cellular responses. PDGF-C appears to be involved in the three stages of wound healing: inflammation, proliferation and remodeling. PDGF-C is involved in fibrotic processes, in which transformation of interstitial fibroblasts into myofibroblasts plus collagen deposition occurs.

References

Li X, et al. (2000) PDGF-C is a new protease-activated ligand for the PDGF alpha-receptor. Nat Cell Biol. 2 (5): 302-9.Ding H, et al. (2004) A specific requirement for PDGF-C in palate formation and PDGFR-alpha signaling. Nat Genet. 36 (10): 1111-6.Choi SJ, et al. (2009) The PDGF-C regulatory region SNP rs28999109 decreases promoter transcriptional activity and is associated with CL/P. European Journal of Human Genetics. 17 (11): 774-84.

NCBI and Uniprot Product Information

• NCBI GI #: 9994187
• NCBI GeneID: 56034
• NCBI Accession #: NP_057289.1
• NCBI GenBank Nucleotide #: NM_016205.2
• UniProt Accession #: Q9NRA1; Q4W5M9; Q9UL22; B4DU34; B9EGR8
• Molecular Weight: 39,029 Da
• NCBI Official Full Name: platelet-derived growth factor C
• NCBI Official Synonym Full Names: platelet derived growth factor C
• NCBI Official Symbol: PDGFC
• NCBI Official Synonym Symbols: SCDGF; FALLOTEIN
• NCBI Protein Information: platelet-derived growth factor C; PDGF-C; VEGF-E; spinal cord-derived growth factor; secretory growth factor-like protein
• UniProt Protein Name: Platelet-derived growth factor C
• Protein Family: Platelet-derived growth factor
• UniProt Gene Name: PDGFC
• UniProt Synonym Gene Names: SCDGF; PDGF-C; SCDGF; PDGFC latent form
• UniProt Entry Name: PDGFC_HUMAN

NCBI Description

The protein encoded by this gene is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a core motif of eight cysteines. This gene product appears to form only homodimers. It differs from the platelet-derived growth factor alpha and beta polypeptides in having an unusual N-terminal domain, the CUB domain. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Sep 2010]

Uniprot Description

PDGFC: Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen and chemoattractant for cells of mesenchymal origin. Required for normal skeleton formation during embryonic development, especially for normal development of the craniofacial skeleton and for normal development of the palate. Required for normal skin morphogenesis during embryonic development. Plays an important role in wound healing, where it appears to be involved in three stages: inflammation, proliferation and remodeling. Plays an important role in angiogenesis and blood vessel development. Involved in fibrotic processes, in which transformation of interstitial fibroblasts into myofibroblasts plus collagen deposition occurs. The CUB domain has mitogenic activity in coronary artery smooth muscle cells, suggesting a role beyond the maintenance of the latency of the PDGF domain. In the nucleus, PDGFC seems to have additional function. Belongs to the PDGF/VEGF growth factor family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 4q32

Cellular Component: Golgi membrane; extracellular space; cell surface; endoplasmic reticulum lumen; cytoplasm; plasma membrane; extracellular region; nucleus

Molecular Function: protein binding; protein homodimerization activity; growth factor activity; platelet-derived growth factor receptor binding

Biological Process: organ morphogenesis; embryonic development; positive regulation of fibroblast proliferation; central nervous system development; transmembrane receptor protein tyrosine kinase activation (dimerization); positive regulation of cell division; platelet-derived growth factor receptor signaling pathway; regulation of peptidyl-tyrosine phosphorylation; positive regulation of DNA replication