Human Repulsive guidance molecule A (RGMA) ELISA Kit | RGMA elisa kit

Human Repulsive guidance molecule A (RGMA) ELISA Kit

Cat. Num. AAA7208752

• Gene Names: RGMA; RGM
• Reactivity: Human
• Synonyms: Repulsive guidance molecule A (RGMA); Human Repulsive guidance molecule A (RGMA) ELISA Kit; RGMA elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of RGMA. No significant cross-reactivity or interference between RGMA and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between RGMA and all the analogues, therefore, cross reaction may still exist in some cases.

• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Assay Type: Quantitative Competitive
• Sensitivity: 1.0 pg/mL
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for RGMA elisa kit

Intended Uses: This RGMA ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human RGMA. This ELISA kit for research use only!

Principle of the Assay: RGMA ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-RGMA antibody and an RGMA-HRP conjugate. The assay sample and buffer are incubated together with RGMA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the RGMA concentration since RGMA from samples and RGMA-HRP conjugate compete for the anti-RGMA antibody binding site. Since the number of sites is limited, as more sites are occupied by RGMA from the sample, fewer sites are left to bind RGMA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The RGMA concentration in each sample is interpolated from this standard curve.

Product Categories/Family for RGMA elisa kit

Neuroscience

NCBI and Uniprot Product Information

• NCBI GI #: 261878467
• NCBI GeneID: 56963
• NCBI Accession #: NP_001159761.1
• NCBI GenBank Nucleotide #: NM_001166289.1
• Molecular Weight: 50,035 Da
• NCBI Official Full Name: repulsive guidance molecule A isoform 2
• NCBI Official Synonym Full Names: repulsive guidance molecule family member a
• NCBI Official Symbol: RGMA
• NCBI Official Synonym Symbols: RGM
• NCBI Protein Information: repulsive guidance molecule A; RGM domain family, member A
• UniProt Protein Name: Repulsive guidance molecule A
• Protein Family: Repulsive guidance molecule
• UniProt Gene Name: RGMA
• UniProt Synonym Gene Names: RGM
• UniProt Entry Name: RGMA_HUMAN

NCBI Description

This gene encodes a member of the repulsive guidance molecule family. The encoded protein is a glycosylphosphatidylinositol-anchored glycoprotein that functions as an axon guidance protein in the developing and adult central nervous system. This protein may also function as a tumor suppressor in some cancers. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009]

Uniprot Description

RGMA: Member of the repulsive guidance molecule (RGM) family that performs several functions in the developing and adult nervous system. Regulates cephalic neural tube closure, inhibits neurite outgrowth and cortical neuron branching, and the formation of mature synapses. Binding to its receptor NEO1/neogenin induces activation of RHOA-ROCK1/Rho-kinase signaling pathway through UNC5B-ARHGEF12/LARG-PTK2/FAK1 cascade, leading to collapse of the neuronal growth cone and neurite outgrowth inhibition. Furthermore, RGMA binding to NEO1/neogenin leads to HRAS inactivation by influencing HRAS1-PTK2/FAK1-AKT1 pathway. It also functions as a bone morphogenetic protein (BMP) coreceptor that may signal through SMAD1, SMAD5, and SMAD8. Belongs to the repulsive guidance molecule (RGM) family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, GPI anchor

Chromosomal Location of Human Ortholog: 15q26.1

Cellular Component: endoplasmic reticulum; plasma membrane

Molecular Function: coreceptor activity

Biological Process: BMP signaling pathway; axon guidance