Nectin-2 Cell Lysate | NECTIN2 cell lysate
Mouse Nectin-2 HEK293 Overexpression Lysate
Gene Names
Nectin2; MPH; Pvr; Pvs; Cd112; Pvrl2; AI325026; AI987993; nectin-2
Applications
Western Blot
Synonyms
Nectin-2; Mouse Nectin-2 HEK293 Overexpression Lysate; Mouse CD112/Nectin-2/PVRL2 HEK293 Cell Lysate (WB positive control); poliovirus receptor-related 2 (herpesvirus entry mediator B); Mouse AI325026 Overexpression Lysate; Mouse AI987993 Overexpression Lysate; Mouse Cd112 Overexpression Lysate; Mouse MPH Overexpression Lysate; Mouse nectin-2 Overexpression Lysate; Mouse Pvr Overexpression Lysate; Mouse Pvs Overexpression Lysate; NECTIN2 cell lysate
Host
HEK293 Cells
Form/Format
1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Sequence Positions
331aa
Sequence
Met1-Gly351
Applicable Applications for NECTIN2 cell lysate
Western Blot (WB)
Application Notes
WB: Use at an assay dependent dilution.
Species
Mouse
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
Sequence Construction
A DNA sequence encoding the mouse PVRL2 (NP_033016.3) extracellular domain (Met 1-Gly 351) was fused with a polyhistidine tag at the C-terminus.
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Preparation and Storage
Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.
Samples are stable for up to twelve months from date of receipt.
Related Product Information for NECTIN2 cell lysate
Cluster of Differentiation 112 (CD112), also known as poliovirus receptor related protein 2 (PVRL2 or PRR2), is a single-pass type I transmembrane glycoprotein belonging to the Immunoglobulin superfamily. CD112 protein also serves as an entry for certain mutant strains of herpes simplex virus and pseudorabies virus, and thus is involved in cell to cell spreading of these viruses. CD112 protein has been identified as the ligand for DNAM-1 (CD226), and the interaction of CD226/CD112 protein can induce NK cell-and CD8+ T cell-mediated cytotoxicity and cytokine secretion. CD112 has been regarded as a critical component in allergic reactions, and accordingly may function as a novel target for anti-allergic therapy.
This Mouse Nectin-2 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of Nectin-2 protein from the overexpression lysate was verified.
This Mouse Nectin-2 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of Nectin-2 protein from the overexpression lysate was verified.
References
Bachelet I, et al. (2006) Mast cell costimulation by CD226/CD112 (DNAM-1/Nectin-2): a novel interface in the allergic process. J Biol Chem. 281(37): 27190-6.Wang L, et al. (2009) Molecular cloning, characterization and three-dimensional modeling of porcine nectin-2/CD112. Vet Immunol Immunopathol. 132(2-4): 257-63.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
50,779 Da
NCBI Official Full Name
nectin-2 isoform 1
NCBI Official Synonym Full Names
nectin cell adhesion molecule 2
NCBI Official Symbol
Nectin2
NCBI Official Synonym Symbols
MPH; Pvr; Pvs; Cd112; Pvrl2; AI325026; AI987993; nectin-2
NCBI Protein Information
nectin-2
UniProt Protein Name
Nectin-2
Protein Family
UniProt Gene Name
Pvrl2
UniProt Synonym Gene Names
Mph; Pvr; Pvs; Herpesvirus entry mediator B; HveB; mHveB
UniProt Entry Name
PVRL2_MOUSE
Uniprot Description
nectin 2: Probable cell adhesion protein. Belongs to the nectin family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Cell adhesion; Membrane protein, integral
Cellular Component: focal adhesion; cell surface; membrane; cell; plasma membrane; integral to membrane; zonula adherens; intercellular junction
Molecular Function: identical protein binding; protein binding; protein homodimerization activity; cell adhesion molecule binding
Biological Process: establishment of mitochondrion localization; acrosome formation; sperm mitochondrion organization and biogenesis; positive regulation of natural killer cell mediated cytotoxicity directed against tumor cell target; positive regulation of immunoglobulin mediated immune response; spermatid nuclear differentiation; cytoskeleton organization and biogenesis; susceptibility to natural killer cell mediated cytotoxicity; virion attachment, binding of host cell surface coreceptor; positive regulation of natural killer cell mediated cytotoxicity; positive regulation of mast cell activation; viral envelope fusion with host membrane; fertilization; cell part morphogenesis; adhesion to host; cell adhesion; homophilic cell adhesion; spermatid development
Research Articles on NECTIN2
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Product Notes
The NECTIN2 pvrl2 (Catalog #AAA8116536) is a Cell Lysate produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 331aa. AAA Biotech's Nectin-2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: Use at an assay dependent dilution. Researchers should empirically determine the suitability of the NECTIN2 pvrl2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Met1-Gly35 1. It is sometimes possible for the material contained within the vial of "Nectin-2, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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